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ZFIN ID: ZDB-FIG-140321-13
Miller et al., 2000 - sucker encodes a zebrafish Endothelin-1 required for ventral pharyngeal arch development. Development (Cambridge, England)   127(17):3815-3828
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 6 suc/et-1 is required for ventral neural crest specification in the arch primordia. Lateral views of whole-mount embryos processed for single color (A-F) or two-color (G,H) in situ hybridization. In all panels, the arches are numbered, and pharyngeal pouches are marked with black dots. (A-B) 24 hours, msxE expression in wild type (A) and suc/et-1 mutant (B). Ventral mesenchymal cells in the first three arches express msxE in wild types, but do not in suc/et-1 mutants. (C-D) 30 hour, gsc expression in wild type (C) and suc/et-1 mutant (D). In wild types, gsc is expressed in separate patches of dorsal and ventral mesenchyme in the second arch. In suc/et-1 mutants, dorsal cells express gsc while ventral cells do not. (E-F) 24 hours, dlx3 expression in wild type (E) and suc/et-1 mutant (F). In wild types at this stage, dlx3 is expressed in ventral but not dorsal mesenchyme in arches one, two, and four. In suc/et-1 mutants, ventral cells fail to express dlx3. (G-H) 32 hours, dlx2 (red) and EphA3 (blue) expression in wild type (G) and suc/et-1 mutant (H). EphA3 expression is ventrally restricted similar to dHAND. In suc/et-1 mutants, EphA3 expression is severely reduced in the first four arches. Fig. 5 should be referred to for the dlx2 defect in suc/et-1 mutants. e, eye; o, otic vesicle. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT standard conditions Prim-5 Not Detected pharyngeal arch 1 dorsal region ISH
Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 pharyngeal arch 1 ventral region ISH
Prim-5 Not Detected pharyngeal arch 2 dorsal region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 pharyngeal arch 2 ventral region ISH
Prim-5 Not Detected pharyngeal arch 3 dorsal region ISH
Prim-5 Not Detected pharyngeal arch 3 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 3 ventral region ISH
Prim-5 Not Detected pharyngeal arch 4 dorsal region ISH
Prim-5 pharyngeal arch 4 mesenchymal cell ISH
Prim-5 pharyngeal arch 4 ventral region ISH
edn1tf216b/tf216b standard conditions Prim-5 Not Detected pharyngeal arch 1 dorsal region ISH
Prim-5 Not Detected pharyngeal arch 1 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 1 ventral region ISH
Prim-5 Not Detected pharyngeal arch 2 dorsal region ISH
Prim-5 Not Detected pharyngeal arch 2 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 2 ventral region ISH
Prim-5 Not Detected pharyngeal arch 3 dorsal region ISH
Prim-5 Not Detected pharyngeal arch 3 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 3 ventral region ISH
Prim-5 Not Detected pharyngeal arch 4 dorsal region ISH
Prim-5 Not Detected pharyngeal arch 4 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 4 ventral region ISH
epha4b WT standard conditions Prim-15 Not Detected pharyngeal arch 1 dorsal region ISH
Prim-15 pharyngeal arch 1 mesenchymal cell ISH
Prim-15 pharyngeal arch 1 ventral region ISH
Prim-15 Not Detected pharyngeal arch 2 dorsal region ISH
Prim-15 pharyngeal arch 2 mesenchymal cell ISH
Prim-15 pharyngeal arch 2 ventral region ISH
Prim-15 Not Detected pharyngeal arch 3 dorsal region ISH
Prim-15 pharyngeal arch 3 mesenchymal cell ISH
Prim-15 pharyngeal arch 3 ventral region ISH
Prim-15 Not Detected pharyngeal arch 4 dorsal region ISH
Prim-15 pharyngeal arch 4 mesenchymal cell ISH
Prim-15 pharyngeal arch 4 ventral region ISH
edn1tf216b/tf216b standard conditions Prim-15 Not Detected pharyngeal arch 1 dorsal region ISH
Prim-15 Not Detected pharyngeal arch 1 mesenchymal cell ISH
Prim-15 Not Detected pharyngeal arch 1 ventral region ISH
Prim-15 Not Detected pharyngeal arch 2 dorsal region ISH
Prim-15 Not Detected pharyngeal arch 2 mesenchymal cell ISH
Prim-15 Not Detected pharyngeal arch 3 ventral region ISH
Prim-15 Not Detected pharyngeal arch 4 dorsal region ISH
Prim-15 Not Detected pharyngeal arch 4 mesenchymal cell ISH
Prim-15 Not Detected pharyngeal arch 4 ventral region ISH
gsc WT standard conditions Prim-15 pharyngeal arch 2 dorsal region ISH
Prim-15 pharyngeal arch 2 mesenchymal cell ISH
Prim-15 pharyngeal arch 2 ventral region ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch 2 dorsal region ISH
Prim-15 pharyngeal arch 2 mesenchymal cell ISH
Prim-15 Not Detected pharyngeal arch 2 ventral region ISH
msx1a WT standard conditions Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 pharyngeal arch 1 ventral region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 pharyngeal arch 2 ventral region ISH
Prim-5 pharyngeal arch 3 mesenchymal cell ISH
Prim-5 pharyngeal arch 3 ventral region ISH
edn1tf216b/tf216b standard conditions Prim-5 Not Detected pharyngeal arch 1 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 1 ventral region ISH
Prim-5 Not Detected pharyngeal arch 2 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 2 ventral region ISH
Prim-5 Not Detected pharyngeal arch 3 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 3 ventral region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
Prim-5 pharyngeal arch 1 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 1 ventral region physical object quality, abnormal
Prim-5 pharyngeal arch 2 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 2 ventral region physical object quality, abnormal
Prim-5 pharyngeal arch 3 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 3 ventral region physical object quality, abnormal
Prim-5 pharyngeal arch 4 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 4 ventral region physical object quality, abnormal
Prim-15 embryonic viscerocranium morphogenesis decreased process quality, abnormal
Prim-15 pharyngeal arch 2 mesenchymal cell physical object quality, abnormal
Prim-15 pharyngeal arch 2 ventral region physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.