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ZFIN ID: ZDB-FIG-140321-12
Miller et al., 2000 - sucker encodes a zebrafish Endothelin-1 required for ventral pharyngeal arch development. Development (Cambridge, England)   127(17):3815-3828
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Observed In:
Stage: Prim-5

Fig. 5 dlx2, dHAND, and suc/et-1 expression in wild-type (A-E,G,I,K) and suc/et-1 mutants (F,H,J,L). In all panels, arches are numbered and when visible, the pharyngeal pouches are marked with black dots. (A) Two-color in situ hybridization with dlx2 in red and dHAND in dark blue in a wild-type embryo at 28 hours. At this stage, dHAND expression marks a ventral subset of dlx2-expressing mesenchymal cells in each of the first four arches. (B) dlx2 expression in horizontal section through arches 2 and 3 at 28 hours. Within each arch, dlx2-expressing cells surround a core of nonexpressing cells. (C) Ventral view of dHAND expression in wholemount embryo at 28 hours. Within each arch, dHAND-expressing cells also surround a central core of non-expressing cells. (D) Ventral view at 28 hours showing suc/et-1 expression, which is strikingly complementary to dHAND expression (compare with C). suc/et-1 expression is detected in a central core of mesenchymal cells in the first two arches, and in arch epithelia, both ventral surface ectoderm and the second pharyngeal pouch. (E-F), Lateral views, at 28 hours, of dlx2 expression in PCR-genotyped wild-type (E) and suc/et-1 mutant (F) whole-mount embryos. In wild types dlx2 is expressed broadly in most if not all postmigratory arch neural crest, and is not expressed in the pharyngeal pouches. suc/et-1 mutant embryos have a reduction of ventral dlx2 expression. (G-H) 28 hours; sagittal sections of dlx2 expression in PCR-genotyped wild-type (G) and suc/et-1 mutant (H) cut through the lateral aspect of arches 1 and 2. In suc/et-1 mutant embryos, ventral cells are present but do not express dlx2 (black arrows). In contrast, suc/et-1 mutant dorsal cells (white arrows) and cells lining the stomodeum express dlx2 at normal levels. (I-L) dHAND expression in whole-mount wild-type (I, K) and suc/et-1 mutant (J,L) embryos at 28 hours. I and J are lateral views, K and L are ventral views. dHAND expression in suc/et-1 mutants is severely reduced in the first two arches (arrows in J and L). dHAND is also normally expressed in the heart, the pectoral fin (data not shown), and a discrete cluster of cells at the arch one/two boundary (arrowhead in L); these domains are not affected in suc/et-1 mutants (K-L and data not shown). e, eye; h, heart; o, otic vesicle; se, surface ectoderm; st, stomodeum. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions Prim-5 Not Detected pharyngeal arch 1 central region ISH
Prim-5 pharyngeal arch 1 dorso-lateral region ISH
Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 pharyngeal arch 1 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 2 central region ISH
Prim-5 pharyngeal arch 2 dorso-lateral region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 pharyngeal arch 2 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 3 central region ISH
Prim-5 pharyngeal arch 3 dorso-lateral region ISH
Prim-5 pharyngeal arch 3 mesenchymal cell ISH
Prim-5 pharyngeal arch 3 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 4 central region ISH
Prim-5 pharyngeal arch 4 dorso-lateral region ISH
Prim-5 pharyngeal arch 4 mesenchymal cell ISH
Prim-5 pharyngeal arch 4 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal pouch ISH
edn1tf216b/tf216b standard conditions Prim-5 Not Detected pharyngeal arch 1 central region ISH
Prim-5 pharyngeal arch 1 dorso-lateral region ISH
Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 1 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 2 central region ISH
Prim-5 pharyngeal arch 2 dorso-lateral region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 2 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 3 central region ISH
Prim-5 pharyngeal arch 3 dorso-lateral region ISH
Prim-5 pharyngeal arch 3 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 3 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 4 central region ISH
Prim-5 pharyngeal arch 4 dorso-lateral region ISH
Prim-5 pharyngeal arch 4 mesenchymal cell ISH
Prim-5 Not Detected pharyngeal arch 4 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal pouch ISH
edn1 WT standard conditions Prim-5 pharyngeal arch 1 central region ISH
Prim-5 pharyngeal arch 1 ecto-epithelial cell ISH
Prim-5 Not Detected pharyngeal arch 1 lateral region ISH
Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 pharyngeal arch 2 central region ISH
Prim-5 pharyngeal arch 2 ecto-epithelial cell ISH
Prim-5 Not Detected pharyngeal arch 2 lateral region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 pharyngeal pouch 2 ISH
hand2 WT standard conditions Prim-5 Not Detected pharyngeal arch ecto-epithelial cell ISH
Prim-5 Not Detected pharyngeal arch 1 central region ISH
Prim-5 Not Detected pharyngeal arch 1 dorso-lateral region ISH
Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 pharyngeal arch 1 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 2 central region ISH
Prim-5 Not Detected pharyngeal arch 2 dorso-lateral region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 pharyngeal arch 2 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 3 central region ISH
Prim-5 Not Detected pharyngeal arch 3 dorso-lateral region ISH
Prim-5 pharyngeal arch 3 mesenchymal cell ISH
Prim-5 pharyngeal arch 3 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 4 central region ISH
Prim-5 Not Detected pharyngeal arch 4 dorso-lateral region ISH
Prim-5 pharyngeal arch 4 mesenchymal cell ISH
Prim-5 pharyngeal arch 4 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal pouch ISH
edn1tf216b/tf216b standard conditions Prim-5 Not Detected pharyngeal arch ecto-epithelial cell ISH
Prim-5 Not Detected pharyngeal arch 1 central region ISH
Prim-5 Not Detected pharyngeal arch 1 dorso-lateral region ISH
Prim-5 pharyngeal arch 1 mesenchymal cell ISH
Prim-5 pharyngeal arch 1 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 2 central region ISH
Prim-5 Not Detected pharyngeal arch 2 dorso-lateral region ISH
Prim-5 pharyngeal arch 2 mesenchymal cell ISH
Prim-5 pharyngeal arch 2 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 3 central region ISH
Prim-5 Not Detected pharyngeal arch 3 dorso-lateral region ISH
Prim-5 pharyngeal arch 3 mesenchymal cell ISH
Prim-5 pharyngeal arch 3 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal arch 4 central region ISH
Prim-5 Not Detected pharyngeal arch 4 dorso-lateral region ISH
Prim-5 pharyngeal arch 4 mesenchymal cell ISH
Prim-5 pharyngeal arch 4 ventro-lateral region ISH
Prim-5 Not Detected pharyngeal pouch ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Prim-5 embryonic viscerocranium morphogenesis decreased process quality, abnormal
Prim-5 pharyngeal arch 1 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 1 ventral region physical object quality, abnormal
Prim-5 pharyngeal arch 2 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 2 ventral region physical object quality, abnormal
Prim-5 pharyngeal arch 3 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 3 ventral region physical object quality, abnormal
Prim-5 pharyngeal arch 4 mesenchymal cell physical object quality, abnormal
Prim-5 pharyngeal arch 4 ventral region physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.