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ZFIN ID: ZDB-FIG-140321-14
Miller et al., 2000 - sucker encodes a zebrafish Endothelin-1 required for ventral pharyngeal arch development. Development (Cambridge, England)   127(17):3815-3828
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 7 suc/et-1 is required for later mesodermal and endodermal patterning in the pharyngeal arches. (A-D) myoD expression at 54 hours in wild types (A,C) and suc/et-1 mutants (B,D). In mutants, the mouth is displaced ventrally, and the ventral myoD-expressing premyogenic condensations in arches 1 and 2 are absent. (E-H) Larval muscles at 5 days detected with the 1025 antibody to muscle myosins in wild types (E,G) and suc/et-1 mutants (F,H). In mutant larvae, ventral muscles are severely mispatterned. The first arch ventral muscles, intermandibularis anterior and posterior (ima and imp), are missing in suc/et-1 mutants, other than a few occasional fibers spanning the midline. The ventral arch two muscles, interhyoideus and hyohyoideus (ih and hh), are dramatically shorter and do not meet in the midline as they do in wild-types (E-F). Dorsal muscles (am, lap, do, ao) appear normal in suc/et-1 mutants. (I-L) shh expression in wild types (I,K) and suc/et-1 mutants (J,L) at 54 hours. (I,J) Anterior views, focused deep to the mouth at the level of the first pharyngeal pouch, (K,L) lateral views. In mutants, expression in the first pharyngeal pouch does not extend as laterally as in wild types (arrows in I and J) and a midline diverticulum has not formed (arrows in K and L). am, adductor mandibulae; ao, adductor operculi; do, dilator operculi; h, heart; ih, interhyoideus; ima, intermandibularis anterior; imp, intermandibularis posterior; hh, hyohyoideus; lap, levator arcus palantini; p, pharynx; sh, sternohyoideus; tv1-4, branchial muscles transversus ventralis 1-4 (see Schilling and Kimmel, 1997 for wild-type muscle description). Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
myod1 WT standard conditions Long-pec interhyoideus ISH
Long-pec intermandibularis ISH
edn1tf216b/tf216b standard conditions Long-pec Not Detected interhyoideus ISH
Long-pec Not Detected intermandibularis ISH
shha WT standard conditions Long-pec pharyngeal pouch 1 ISH
edn1tf216b/tf216b standard conditions Long-pec pharyngeal pouch 1 ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab-A4.1025 IHC WT standard conditions Day 5 adductor mandibulae
IHC Day 5 adductor operculi
IHC Day 5 dilator operculi
IHC Day 5 hyohyoideus
IHC Day 5 interhyoideus
IHC Day 5 levator arcus palatini
IHC Day 5 sternohyoid
IHC Day 5 ventral intermandibularis anterior
IHC Day 5 ventral intermandibularis posterior
IHC edn1tf216b/tf216b standard conditions Day 5 adductor mandibulae
IHC Day 5 adductor operculi
IHC Day 5 dilator operculi
IHC Day 5 levator arcus palatini
IHC Day 5 sternohyoid
IHC Day 5 Not Detected ventral intermandibularis anterior
IHC Day 5 Not Detected ventral intermandibularis posterior
Phenotype Details
Fish Conditions Stage Phenotype
edn1tf216b/tf216b standard conditions Long-pec branchiomeric skeletal muscle development decreased process quality, abnormal
Long-pec interhyoideus physical object quality, abnormal
Long-pec pharyngeal pouch 1 physical object quality, abnormal
Long-pec stomodeum mislocalised ventrally, abnormal
Long-pec ventral intermandibularis anterior physical object quality, abnormal
Long-pec ventral intermandibularis posterior physical object quality, abnormal
Day 5 branchiomeric skeletal muscle development decreased process quality, abnormal
Day 5 head lacks all parts of type ventral intermandibularis anterior, abnormal
Day 5 head lacks all parts of type ventral intermandibularis posterior, abnormal
Day 5 hyohyoideus decreased length, abnormal
Day 5 interhyoideus decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright.