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ZFIN ID: ZDB-FIG-131009-25
Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling. BMC Developmental Biology   13:31 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 75%-epiboly to 1-4 somites

Fig. 5

Gene expression changes upon Zic2 knockdown can be rescued by RA treatment and is mimicked by Aldh1a2 knockdown. mRNA in situ analysis of meis3(A-D, I-J), hoxd4a(E-H, K-L) and aldh1a2(M-N). Uninjected embryos were treated with DMSO (A, E) or 5 nm RA (B, F) and compared to Zic2a;Zic2bMO-injected embryos treated with DMSO (C, G) or 5 nm RA (D, H). The decrease in meis3 and hoxd4a observed in Zic morpholino-injected embryos is rescued by treatment with 5 nm RA. Aldh1a2MO-injected embryos (J, L) show a decrease in meis3 and hoxd4a when compared to controls (I, K). In cyp26a1 (giraffe) mutants (N), aldh1a2 levels remain unchanged compared to WT siblings (M). Images are dorsal views of 10.5 hpf (A-L) and 8 hpf (M-N) embryos oriented with anterior to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aldh1a2 WT standard conditions 75%-epiboly margin ISH
cyp26a1rw716/rw716 standard conditions 75%-epiboly margin ISH
hoxd4a WT control 1-4 somites hindbrain neural plate ISH
WT chemical treatment: all-trans-retinoic acid 1-4 somites hindbrain neural plate ISH
WT + MO2-aldh1a2 standard conditions 1-4 somites hindbrain neural plate ISH
WT + MO2-zic2b + MO3-zic2a + MO4-tp53 chemical treatment: all-trans-retinoic acid 1-4 somites hindbrain neural plate ISH
WT + MO2-zic2b + MO3-zic2a + MO4-tp53 standard conditions 1-4 somites hindbrain neural plate ISH
meis3 WT control 1-4 somites hindbrain neural plate ISH
WT chemical treatment: all-trans-retinoic acid 1-4 somites hindbrain neural plate ISH
WT + MO2-aldh1a2 standard conditions 1-4 somites hindbrain neural plate ISH
WT + MO2-zic2b + MO3-zic2a + MO4-tp53 chemical treatment: all-trans-retinoic acid 1-4 somites hindbrain neural plate ISH
WT + MO2-zic2b + MO3-zic2a + MO4-tp53 standard conditions 1-4 somites hindbrain neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.