ZFIN ID: ZDB-FIG-131009-21
Drummond et al., 2013 - The role of Zic transcription factors in regulating hindbrain retinoic acid signaling. BMC Developmental Biology   13:31 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 30%-epiboly to 14-19 somites

Fig. 1

Temporal and spatial analysis of zic transcription factors and key RA synthesis and degradation genes.aldh1a2 expression is restricted to the embryonic margin at 5 hpf (A). By 8-10 hpf aldh1a2 is observed in the lateral plate mesoderm (B, C, C′). At 18 hpf, aldh1a2 is expressed in the dorsal retina and anterior somites (D). cyp26a1 is expressed in the embryonic margin and the presumptive anterior neural ectoderm at 5-8 hpf (E, F). By 10-18 hpf cyp26a1 is expressed in presumptive forebrain, midbrain, anterior hindbrain, retina, and part of the tailbud (G, G′, H). zic1 is not detectable until 8-10 hpf (75% epiboly), when it is expressed within the presumptive anterior neural tissue (I, J, K, K′). By 18 hpf, zic1 is expressed strongly in the telencephalon, midbrain-hindbrain boundary, dorsal hindbrain and spinal cord (L). zic2a initiates earlier, with dorsally-restricted expression at 5 hpf (50% epiboly) (M). By 8-10 hpf zic2a becomes anteriorly restricted within presumptive anterior neural tissue with additional midline expression (N, O, O′). By 18 hpf, zic2a is within the anterior forebrain, ventral eye, dorsal hindbrain and spinal cord (P). Initially, zic2b is expressed in a broad domain encompassing the dorsal side of the embryo (Q; 5 hpf). This broad expression is maintained at 8 hpf (R) and 10-18 hpf where expression is strongest within the eye, midbrain-hindbrain boundary, and presumptive hindbrain (S, S′, T). zic3 is also dorsally restricted at 5-8 hpf (U, V). At 10-18 hpf, zic3 is within the presumptive telencephalon, posterior forebrain, midbrain-hindbrain boundary and dorsal hindbrain, and within the tailbud (W, W′, X). Images are dorsal views with anterior to top (A-C, E-G, I-K, M-O, Q-S, U-W) or lateral views with anterior to left (C′, D, G′, H, K′, L, O′, P, S′, T, W′, X). hpf: hours post fertilization.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
aldh1a2 WT standard conditions 30%-epiboly margin ISH
75%-epiboly mesoderm ISH
Bud mesoderm ISH
14-19 somites optic vesicle dorsal region ISH
14-19 somites somite ISH
cyp26a1 WT standard conditions 30%-epiboly DEL ISH
30%-epiboly margin ISH
75%-epiboly margin ISH
75%-epiboly neuroectoderm anterior region ISH
Bud forebrain neural plate ISH
Bud hindbrain neural plate anterior region ISH
Bud midbrain neural plate ISH
Bud tail bud ISH
14-19 somites notochord posterior side ISH
14-19 somites optic vesicle ISH
14-19 somites tail bud ISH
zic1 WT standard conditions 30%-epiboly Not Detected anatomical structure ISH
75%-epiboly neuroectoderm anterior region ISH
Bud neuroectoderm anterior region ISH
14-19 somites hindbrain dorsal region ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites spinal cord neural tube ISH
14-19 somites telencephalon ISH
zic2a WT standard conditions 30%-epiboly whole organism dorsal region ISH
75%-epiboly axis ISH
75%-epiboly neuroectoderm anterior region ISH
Bud axis ISH
Bud neuroectoderm anterior region ISH
14-19 somites forebrain anterior region ISH
14-19 somites hindbrain dorsal region ISH
14-19 somites optic vesicle ventral region ISH
14-19 somites spinal cord neural tube ISH
zic2b WT standard conditions 30%-epiboly whole organism dorsal region ISH
75%-epiboly whole organism dorsal region ISH
Bud hindbrain neural plate ISH
Bud midbrain hindbrain boundary neural plate ISH
Bud optic primordium ISH
14-19 somites hindbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites optic vesicle ISH
zic3 WT standard conditions 30%-epiboly whole organism dorsal region ISH
75%-epiboly whole organism dorsal region ISH
Bud forebrain neural plate ISH
Bud hindbrain neural plate dorsal region ISH
Bud midbrain hindbrain boundary neural plate ISH
Bud tail bud ISH
14-19 somites forebrain posterior region ISH
14-19 somites hindbrain dorsal region ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites tail bud ISH
14-19 somites telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.