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ZFIN ID: ZDB-FIG-130906-21
Armant et al., 2013 - Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos. Developmental Biology   380(2):351-62 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: Prim-5

Fig. 4

Assessment of TR gene expression by RNAseq. (A) Number of genes (white bars) and TRs (dashed bars) expressed at two different developmental stages and two adult body parts as determined by RNAseq. The total number of TR loci quantified by transcript counting over all stages is indicated as “Collapsed”. (B) Quantification of the total number of genes detected at 24 hpf by RNAseq in function of the sequencing depth. The number of detected genes is indicated as the mean from 3 biological replicates. (C) Level of expression of transcripts represented as FPKM from a selection of genes known to be expressed in the epiphysis (crx, otx5, nr2e3, aannt2), the retina (pax6b, hmx4) or tectum and retina (mycn and mych). The relative expression is indicated as the mean of FPKM from biological triplicates. (D) RNA in situ expression data of the selected transcripts at 24 hpf. (*: epiphysis; arrow head: optic tectum.)

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aff2 AB standard conditions Prim-5 intermediate cell mass of mesoderm ISH
btbd6b AB standard conditions Prim-5 somite ISH
dpf2 AB standard conditions Prim-5 epiphysis ISH
drl AB standard conditions Prim-5 intermediate cell mass of mesoderm ISH
foxo6b AB standard conditions Prim-5 telencephalon ISH
hlfb AB standard conditions Prim-5 hypothalamus ISH
klhl4 AB standard conditions Prim-5 intermediate cell mass of mesoderm ISH
klhl41a AB standard conditions Prim-5 somite ISH
myt1la AB standard conditions Prim-5 telencephalon ISH
nfil3-6 AB standard conditions Prim-5 epiphysis ISH
nfixb AB standard conditions Prim-5 telencephalon ISH
nkx2.4a AB standard conditions Prim-5 hypothalamus ISH
pbrm1 AB standard conditions Prim-5 somite ISH
pbx3b AB standard conditions Prim-5 telencephalon ISH
six6b AB standard conditions Prim-5 hypothalamus ISH
tbx21 AB standard conditions Prim-5 telencephalon ISH
tnrc18 AB standard conditions Prim-5 hypothalamus ISH
znf296 AB standard conditions Prim-5 telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 380(2), Armant, O., März, M., Schmidt, R., Ferg, M., Diotel, N., Ertzer, R., Bryne, J.C., Yang, L., Baader, I., Reischl, M., Legradi, J., Mikut, R., Stemple, D., Ijcken, W.V., van der Sloot, A., Lenhard, B., Strähle, U., and Rastegar, S., Genome-wide, whole mount in situ analysis of transcriptional regulators in zebrafish embryos, 351-62, Copyright (2013) with permission from Elsevier. Full text @ Dev. Biol.