ZFIN ID: ZDB-FIG-130828-11
Sacilotto et al., 2013 - Analysis of Dll4 regulation reveals a combinatorial role for Sox and Notch in arterial development. Proceedings of the National Academy of Sciences of the United States of America   110(29):11893-8 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. S8

(AD) Whole-mount in situ hybridization of arterial markers on control, rbpj, double sox7;sox18, and triple sox7;sox18;rbpj MO at 26 hpf. Triple sox7; sox18;rbpj knockdown results in the loss of all of the arterial markers analyzed. Black arrowhead shows dorsal aorta. White arrowhead indicates where dorsal aorta should be. Black arrow indicates posterior cardinal vein, and white bracket indicates single axial vessel/expanded posterior cardinal vein. Black bracket indicates notochord. Probes used are depicted in the bottom left of each picture. Values on the bottom right indicate number of embryos with the predominant and displayed phenotype/total number of embryos analyzed. In all cases, 0.125 pmol of sox7 and sox18 and 0.15 pmol of rbpj MO were used. (E–K) Whole-mount in situ hybridization of arterial (A–F) and venous (G) markers on control and dll4 MO-injected WT zebrafish embryos at 26 hpf. Probes are depicted in the bottom left. Values on the bottom right indicate number of embryos with the predominant and displayed phenotype per total number of embryos. Six nanograms of dll4 MO were used.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlc WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO1-dll4 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
flt1 WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO1-dll4 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected artery ISH
Prim-5 Not Detected dorsal aorta ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
flt4 WT control Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
WT + MO1-dll4 standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
hey2 WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO1-dll4 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected artery ISH
Prim-5 Not Detected dorsal aorta ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
notch1b WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO1-dll4 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected artery ISH
Prim-5 Not Detected dorsal aorta ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
notch3 WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO1-dll4 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 Not Detected artery ISH
Prim-5 Not Detected dorsal aorta ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected artery ISH
Prim-5 Not Detected dorsal aorta ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-dll4 standard conditions Prim-5 blood vessel development disrupted, abnormal
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery development arrested, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA