ZFIN ID: ZDB-FIG-130828-5
Sacilotto et al., 2013 - Analysis of Dll4 regulation reveals a combinatorial role for Sox and Notch in arterial development. Proceedings of the National Academy of Sciences of the United States of America   110(29):11893-8 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage Range: Prim-5 to Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Prim-15

Fig. 4

Combinatorial knockdown of sox7, sox18, and rbpj in zebrafish results in ablation of both Dll4in3:GFP and endogenous dll4 expression. (A) Analysis of GFP expression in scrambled, rbpj, double sox7;sox18, and triple sox7;sox18;rbpj MO-injected tg(Dll4in3:GFP) embryos at 34 hpf. (B) Analysis of endogenous dll4 gene expression in scrambled, rbpj, double sox7;sox18, and triple sox7;sox18;rbpj MO-injected WT zebrafish embryos at 26 hpf. dll4 expression was detected by in situ hybridization. Values on the bottom right indicate number of embryos with the predominant and displayed phenotype per total number of embryos analyzed. In all cases, 0.125 pmol of sox7 and sox18 and 0.15 pmol of rbpj MO were used. *, highlights ectopic neural expression; arrowhead shows dorsal aorta. See also Fig. S6.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dll4 WT control Prim-5 artery ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 artery ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected artery ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
EGFP lcr1Tg control Prim-15 artery IFL
lcr1Tg + MO4-rbpja,rbpjb standard conditions Prim-15 artery IFL
lcr1Tg + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-15 Not Detected artery IFL
lcr1Tg + MO5-sox18 + MO5-sox7 standard conditions Prim-15 artery IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-rbpja,rbpjb standard conditions Prim-5 angiogenic sprout increased amount, abnormal
Prim-5 blood vessel endothelial cell proliferation involved in sprouting angiogenesis process quality, abnormal
Prim-5 intersegmental vessel structure, abnormal
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery development arrested, abnormal
Prim-5 whole organism lacks all parts of type dorsal aorta, abnormal
lcr1Tg + MO4-rbpja,rbpjb standard conditions Prim-5 angiogenic sprout increased amount, abnormal
Prim-5 blood vessel endothelial cell proliferation involved in sprouting angiogenesis process quality, abnormal
Prim-5 intersegmental vessel structure, abnormal
lcr1Tg + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-15 artery development arrested, abnormal
Prim-15 whole organism lacks all parts of type dorsal aorta, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA