ZFIN ID: ZDB-FIG-130828-6
Sacilotto et al., 2013 - Analysis of Dll4 regulation reveals a combinatorial role for Sox and Notch in arterial development. Proceedings of the National Academy of Sciences of the United States of America   110(29):11893-8 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 10-13 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: 10-13 somites to Long-pec

Fig. 5

Triple sox7;sox18;rbpj knockdown results in the loss of a detectable dorsal aorta. (A) Analysis of GFP expression in tg(fli1a:GFP) zebrafish embryos after injection of control (Upper) and triple sox7;sox18;rbpj MO (Lower) during the migration of angioblasts from the lateral plate mesoderm toward the midline and coalescence into a vascular cord. At 26 hpf, the dorsal aorta (red bracket) and posterior cardinal vein (blue bracket) can be detected in the control MO-injected embryo but not in the triple MO (white bracket highlights single axial vessel). See also Fig. S7 A and B. (B) Lateral view (Upper) and optical transversal sections (Lower, location indicated by white bar) of control, rbpj, sox7;sox18, and sox7;sox18;rbpj MO-injected fli1a:EGFP embryos at 2 dpf. See also Movies S1, S2, S3, and S4 for 3D animations of each embryo. (CE) Whole-mount in situ hybridization of venous (C) and arterial (D and E) markers on control, rbpj, double sox7;sox1,8 and triple sox7;sox18;rbpj MO at 26 hpf. See also Fig. S8 A–D. Black arrowhead, dorsal aorta; white arrowhead, where dorsal aorta should be; black arrow, posterior cardinal vein; white bracket, single axial vessel; black bracket, notochord. Probes used are depicted in the bottom left of each picture. Values on the bottom right indicate number of embryos with the predominant and displayed phenotype per total number of embryos analyzed. In all cases, 0.125 pmol of sox7 and sox18 and 0.15 pmol of rbpj MO were used.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlc WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected axial blood vessel ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
efnb2a WT control Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 Not Detected axial blood vessel ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery ISH
Prim-5 dorsal aorta ISH
EGFP y1Tg control 10-13 somites lateral plate mesoderm embryonic blood vessel endothelial progenitor cell IFL
14-19 somites vascular cord IFL
20-25 somites vasculature IFL
Prim-5 vasculature IFL
Long-pec vasculature IFL
y1Tg + MO4-rbpja,rbpjb standard conditions Long-pec vasculature IFL
y1Tg + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions 10-13 somites lateral plate mesoderm embryonic blood vessel endothelial progenitor cell IFL
14-19 somites vascular cord IFL
20-25 somites vasculature IFL
Prim-5 vasculature IFL
Long-pec vasculature IFL
y1Tg + MO5-sox18 + MO5-sox7 standard conditions Long-pec vasculature IFL
flt4 WT control Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
WT + MO4-rbpja,rbpjb standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 axial blood vessel ISH
WT + MO5-sox18 + MO5-sox7 standard conditions Prim-5 posterior cardinal vein ISH
Prim-5 vein ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions Prim-5 artery development arrested, abnormal
Prim-5 axial blood vessel decreased amount, abnormal
Prim-5 whole organism lacks all parts of type dorsal aorta, abnormal
y1Tg + MO4-rbpja,rbpjb standard conditions Long-pec axial blood vessel amount, normal
Long-pec blood vessel development disrupted, abnormal
y1Tg + MO4-rbpja,rbpjb + MO5-sox18 + MO5-sox7 standard conditions 10-13 somites angioblast cell migration from lateral mesoderm to midline process quality, normal
14-19 somites angioblast cell migration from lateral mesoderm to midline process quality, normal
14-19 somites vascular cord development process quality, normal
14-19 somites vascular cord present, normal
20-25 somites vascular cord present, normal
Prim-5 artery development arrested, abnormal
Prim-5 axial blood vessel decreased amount, abnormal
Long-pec artery development arrested, abnormal
Long-pec axial blood vessel decreased amount, abnormal
y1Tg + MO5-sox18 + MO5-sox7 standard conditions Long-pec axial blood vessel amount, normal
Long-pec blood vessel development disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA