ZFIN ID: ZDB-FIG-120816-8
Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development. Development (Cambridge, England)   139(17):3232-3241 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3 Loss of p53 does not rescue sbds organogenesis defects. (A) The body curvature observed in SbdsATG MO-injected zebrafish embryos is attenuated in SbdsATG/p53ATG MO-injected embryos at 48 hpf. (B) The p53 transcriptional targets Δ113p53 and p21 are induced upon injection of SbdsATG MO, as assessed by semi-quantitative PCR; co-injection of p53ATG MO abrogates this response. Error bars indicate s.e.m. (C) The 72-hpf pancreatic progenitor phenotype induced by the SbdsATG MO is not rescued by co-injection of p53ATG MO (arrows). (D) The absence of mpo-expressing neutrophils in the intermediate cell mass of SbdsATG/p53ATG MO-injected embryos was detected at 24 hpf (arrowheads). (E) Co-injection of p53Spl MO and SbdsATG MO into ptf1a:eGFP;ins:mCherry embryos does not rescue the defective expansion of pancreatic progenitor cells (arrows). (F) Genetic loss of p53 in p53zdf1/zdf1 embryos does not rescue the SbdsATG MO-induced neutrophil phenotype, as assessed by in situ hybridization for mpo at 24 hpf (arrowheads). (G) The SbdsATG MO-induced pancreatic progenitor defect is not rescued in p53zdf1/zdf1;ptf1a:eGFP;ins:mCherry embryos at 72 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdkn1a WT control Protruding-mouth Not Detected whole organism RTPCR
WT + MO4-sbds standard conditions Protruding-mouth whole organism RTPCR
WT + MO4-sbds + MO4-tp53 standard conditions Protruding-mouth whole organism RTPCR
WT + MO4-tp53 standard conditions Protruding-mouth Not Detected whole organism RTPCR
EGFP jh1Tg; jh2Tg control Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
jh1Tg; jh2Tg + MO3-tp53 + MO4-sbds standard conditions Protruding-mouth pancreas IFL
jh1Tg; jh2Tg + MO4-sbds standard conditions Protruding-mouth pancreas IFL
jh1Tg; jh2Tg + MO4-sbds + MO4-tp53 standard conditions Protruding-mouth pancreas IFL
jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
mCherry jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
jh1Tg; jh2Tg + MO3-tp53 + MO4-sbds standard conditions Protruding-mouth endocrine pancreas IFL
jh1Tg; jh2Tg + MO4-sbds standard conditions Protruding-mouth endocrine pancreas IFL
jh1Tg; jh2Tg + MO4-sbds + MO4-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
mpx WT control Prim-5 intermediate cell mass of mesoderm neutrophil ISH
WT + MO4-sbds standard conditions Prim-5 Not Detected intermediate cell mass of mesoderm neutrophil ISH
WT + MO4-sbds + MO4-tp53 standard conditions Prim-5 Not Detected intermediate cell mass of mesoderm neutrophil ISH
WT + MO4-tp53 standard conditions Prim-5 intermediate cell mass of mesoderm neutrophil ISH
tp53 WT control Protruding-mouth Not Detected whole organism RTPCR
WT + MO4-sbds standard conditions Protruding-mouth whole organism RTPCR
WT + MO4-sbds + MO4-tp53 standard conditions Protruding-mouth whole organism RTPCR
WT + MO4-tp53 standard conditions Protruding-mouth Not Detected whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-sbds standard conditions Prim-5 intermediate cell mass of mesoderm neutrophil absent, abnormal
Prim-5 post-vent region curved, abnormal
WT + MO4-sbds + MO4-tp53 standard conditions Prim-5 intermediate cell mass of mesoderm neutrophil absent, abnormal
Prim-5 post-vent region morphology, normal
WT + MO4-tp53 standard conditions Prim-5 intermediate cell mass of mesoderm neutrophil present, normal
Prim-5 whole organism morphology, normal
jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth whole organism morphology, normal
jh1Tg; jh2Tg + MO3-tp53 + MO4-sbds standard conditions Protruding-mouth endocrine pancreas decreased size, abnormal
Protruding-mouth endocrine pancreas morphology, normal
Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region morphology, normal
jh1Tg; jh2Tg + MO4-sbds standard conditions Protruding-mouth endocrine pancreas decreased size, abnormal
Protruding-mouth endocrine pancreas morphology, normal
Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
jh1Tg; jh2Tg + MO4-sbds + MO4-tp53 standard conditions Protruding-mouth endocrine pancreas decreased size, abnormal
Protruding-mouth endocrine pancreas morphology, normal
Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region morphology, normal
jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth whole organism morphology, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development