FIGURE

Fig. 3

ID
ZDB-FIG-120816-8
Publication
Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development
Other Figures
All Figure Page
Back to All Figure Page
Fig. 3

Loss of p53 does not rescue sbds organogenesis defects. (A) The body curvature observed in SbdsATG MO-injected zebrafish embryos is attenuated in SbdsATG/p53ATG MO-injected embryos at 48 hpf. (B) The p53 transcriptional targets Δ113p53 and p21 are induced upon injection of SbdsATG MO, as assessed by semi-quantitative PCR; co-injection of p53ATG MO abrogates this response. Error bars indicate s.e.m. (C) The 72-hpf pancreatic progenitor phenotype induced by the SbdsATG MO is not rescued by co-injection of p53ATG MO (arrows). (D) The absence of mpo-expressing neutrophils in the intermediate cell mass of SbdsATG/p53ATG MO-injected embryos was detected at 24 hpf (arrowheads). (E) Co-injection of p53Spl MO and SbdsATG MO into ptf1a:eGFP;ins:mCherry embryos does not rescue the defective expansion of pancreatic progenitor cells (arrows). (F) Genetic loss of p53 in p53zdf1/zdf1 embryos does not rescue the SbdsATG MO-induced neutrophil phenotype, as assessed by in situ hybridization for mpo at 24 hpf (arrowheads). (G) The SbdsATG MO-induced pancreatic progenitor defect is not rescued in p53zdf1/zdf1;ptf1a:eGFP;ins:mCherry embryos at 72 hpf.

Expression Data
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development