ZFIN ID: ZDB-FIG-120816-7
Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development. Development (Cambridge, England)   139(17):3232-3241 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Prim-5 to Day 5

Fig. 2 sbds knockdown in zebrafish embryos recapitulates human SDS organogenesis phenotypes. (A) At 24 hpf, loss of neutrophils in SbdsATG MO-injected embryos is revealed by in situ hybridization for the neutrophil marker mpo. mpo-positive cells were absent from both the anterior lateral mesoderm and the intermediate cell mass (top and bottom arrows, respectively). (B) At 120 hpf, abnormal cartilage of the gill arches and cerotohyal (ch) cartilage and bone (arrowheads) were observed in SbdsATG MO-injected embryos. (C) Visualization of ptf1-expressing pancreatic progenitor cells and differentiated beta cells in 72-hpf ptf1a:eGFP;ins:mCherry embryos (arrows). SbdsATG MO-injected embryos showed defects in ptf1-expressing pancreatic progenitors (green), whereas beta cells in the principal islet appeared normal (red). (D) Quantification of the volume of ptf1a:eGFP-expressing cells reveals an almost 75% reduction of pancreatic volume in SbdsATG MO-injected embryos. Error bars indicate s.e.m. (E) Immunofluorescence of phospho-histone H3 (pHH3, blue) marks proliferation in the exocrine pancreatic mass. Proliferation was decreased in the SbdsATG MO-injected embryos at 72 hpf. (F) High-resolution images of the pancreatic islet using gcga:eGFPia1;ins:mCherry double-transgenic zebrafish reveal normal islet architecture with peripheral alpha cells (green) and central beta cells (red) in SbdsATG MO-injected embryos at 72 hpf.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP jh1Tg; jh2Tg control Protruding-mouth pancreas somatic stem cell IFL
jh1Tg; jh2Tg + MO4-sbds standard conditions Protruding-mouth pancreas somatic stem cell IFL
mCherry jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
jh1Tg; jh2Tg + MO4-sbds standard conditions Protruding-mouth endocrine pancreas IFL
mpx WT control Prim-5 anterior lateral mesoderm neutrophil ISH
Prim-5 intermediate cell mass of mesoderm neutrophil ISH
WT + MO4-sbds standard conditions Prim-5 Not Detected anterior lateral mesoderm neutrophil ISH
Prim-5 Not Detected intermediate cell mass of mesoderm neutrophil ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-sbds standard conditions Prim-5 anterior lateral mesoderm neutrophil absent, abnormal
Prim-5 blood vasculature morphology, normal
Prim-5 erythroid progenitor cell present, normal
Prim-5 intermediate cell mass of mesoderm neutrophil absent, abnormal
Prim-5 macrophage normal amount, normal
Prim-5 neutrophil aggregation disrupted, abnormal
Day 5 ceratobranchial cartilage morphology, abnormal
Day 5 ceratohyal cartilage morphology, abnormal
Day 5 opercle decreased thickness, abnormal
Day 5 opercle shape, abnormal
jh1Tg; jh2Tg + MO4-sbds standard conditions Protruding-mouth endocrine pancreas decreased size, abnormal
Protruding-mouth endocrine pancreas pancreatic A cell localized, normal
Protruding-mouth endocrine pancreas pancreatic B cell localized, normal
Protruding-mouth endocrine pancreas position, normal
Protruding-mouth pancreas decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development