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ZFIN ID: ZDB-FIG-120816-10
Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development. Development (Cambridge, England)   139(17):3232-3241 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 6 The pes mutant phenocopies SbdsATG MO-induced p53-independent defects in pancreas development. (A) In situ hybridization at 24 hpf demonstrates pes expression in the eye and central nervous system. At 72 hpf, pes expression is detected in endoderm, including the developing pancreas. (B) The peshi2Tg/hi2Tg phenotype includes axis defects (tail curvature), a small dark head and a small eye. (C) Semi-quantitative RT-PCR of peshi2Tg/hi2Tg embryos reveals activation of the p53 target genes Δ113p53 (24-fold, P<0.0001) and p21 (2.4-fold, P<0.01). Error bars indicate s.e.m. (D) Loss of p53 does not rescue axis defects in peshi2Tg/hi2Tg embryos. Representative images of siblings (sib) generated either from a peshi2Tg/WT incross and injected with or without (Mock) p53ATG MO or p53Spl MO, or from a peshi2Tg/WT;p53zdf1/zdf1 incross. Asterisks indicate siblings displaying the pes phenotype. See supplementary material Fig. S6 for quantification. (E) peshi2Tg/hi2Tg embryos exhibit a p53-independent defect in expansion of ptf1-expressing pancreatic progenitor cells. The pancreatic progenitor defect in 72-hpf peshi2Tg/hi2Tg;ptf1a:eGFP;ins:mCherry embryos is not rescued by p53 MO-mediated knockdown, nor by genetic inactivation of p53 as assessed by incross of peshi2Tg/WT;p53zdf1/zdf1;ptf1a:eGFP;ins:mCherry fish.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdkn1a WT control Protruding-mouth Not Detected whole organism RTPCR
peshi2Tg/hi2Tg standard conditions Protruding-mouth Not Detected whole organism RTPCR
EGFP peshi2Tg/hi2Tg; jh1Tg; jh2Tg control Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
peshi2Tg/hi2Tg; jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
peshi2Tg/hi2Tg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
peshi2Tg; jh1Tg; jh2Tg control Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
peshi2Tg; jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
peshi2Tg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
tp53zdf1/zdf1; peshi2Tg/hi2Tg; jh1Tg; jh2Tg control Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
tp53zdf1/zdf1; peshi2Tg; jh1Tg; jh2Tg control Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
mCherry peshi2Tg/hi2Tg; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
peshi2Tg/hi2Tg; jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
peshi2Tg/hi2Tg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
peshi2Tg; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
peshi2Tg; jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
peshi2Tg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth endocrine pancreas IFL
tp53zdf1/zdf1; peshi2Tg/hi2Tg; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
tp53zdf1/zdf1; peshi2Tg; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
pes WT standard conditions Prim-5 central nervous system ISH
Prim-5 eye ISH
Protruding-mouth endoderm ISH
Protruding-mouth pancreas ISH
tp53 WT control Protruding-mouth Not Detected whole organism RTPCR
peshi2Tg/hi2Tg standard conditions Protruding-mouth whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
peshi2Tg/hi2Tg standard conditions Protruding-mouth eye decreased size, abnormal
Protruding-mouth head decreased size, abnormal
Protruding-mouth head opacity, abnormal
Protruding-mouth post-vent region curved, abnormal
peshi2Tg/hi2Tg; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
peshi2Tg/hi2Tg; jh1Tg; jh2Tg + MO3-tp53 standard conditions Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
peshi2Tg/hi2Tg; jh1Tg; jh2Tg + MO4-tp53 standard conditions Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
tp53zdf1/zdf1; peshi2Tg/hi2Tg; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth post-vent region curved, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development