ZFIN ID: ZDB-FIG-120816-11 |
Genes: | |
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Fish: | |
Knockdown Reagents: | |
Anatomical Terms: | |
Stage Range: | Prim-5 to Protruding-mouth |
Fish: | |
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Knockdown Reagents: | |
Observed In: | |
Stage Range: | Prim-5 to Protruding-mouth |
Fig. 7 MO knockdown of pes phenocopies SbdsATG MO-induced p53-independent defects in pancreas development. (A) Injection of PesATG MO into p53zdf1/zdf1 embryos results in a dose-dependent defect in pancreatic progenitor expansion, as assessed in 72-hpf ptf1a:eGFP;ins:mCherry embryos. (B) Quantification of three independent experiments scored for the percentage of embryos with wild-type pancreas following injection of PesATG MO into a p53zdf1/zdf1;ptf1a:eGFPjh1;ins:mCherryjh2 background. (C) The gross appearance of PesATG MO-injected embryos at 72 hpf includes a small head and eye but normal body axis. (D) Microarray quantification of pes transcript levels indicates an increase in pes expression in SbdsATG MO-injected p53zdf1/zdf1 embryos. (E) Increased abundance of pes transcripts in SbdsATG MO-injected p53zdf1/zdf1 embryos is confirmed by in situ hybridization. Error bars indicate s.e.m. |
Gene | Antibody | Fish | Conditions | Stage | Anatomy | Assay |
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EGFP | tp53zdf1/zdf1; jh1Tg; jh2Tg | control | Protruding-mouth | central nervous system | IFL | |
Protruding-mouth | pancreas | IFL | ||||
Protruding-mouth | pancreas somatic stem cell | IFL | ||||
tp53zdf1/zdf1; jh1Tg; jh2Tg + MO1-pes | standard conditions | Protruding-mouth | central nervous system | IFL | ||
mCherry | tp53zdf1/zdf1; jh1Tg; jh2Tg | control | Protruding-mouth | endocrine pancreas | IFL | |
tp53zdf1/zdf1; jh1Tg; jh2Tg + MO1-pes | standard conditions | Protruding-mouth | endocrine pancreas | IFL | ||
pes | tp53zdf1/zdf1 | control | Prim-5 | eye | ISH | |
Prim-5 | midbrain | ISH | ||||
Long-pec | endoderm | ISH | ||||
Long-pec | pancreas | ISH | ||||
tp53zdf1/zdf1 + MO4-sbds | standard conditions | Prim-5 | eye | ISH | ||
Prim-5 | midbrain | ISH | ||||
Prim-5 | somite | ISH | ||||
Long-pec | endoderm | ISH | ||||
Long-pec | head | ISH | ||||
Long-pec | somite | ISH |
Acknowledgments: | ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development |
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