ZFIN ID: ZDB-FIG-120816-11
Provost et al., 2012 - Ribosomal biogenesis genes play an essential and p53-independent role in zebrafish pancreas development. Development (Cambridge, England)   139(17):3232-3241 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 7 MO knockdown of pes phenocopies SbdsATG MO-induced p53-independent defects in pancreas development. (A) Injection of PesATG MO into p53zdf1/zdf1 embryos results in a dose-dependent defect in pancreatic progenitor expansion, as assessed in 72-hpf ptf1a:eGFP;ins:mCherry embryos. (B) Quantification of three independent experiments scored for the percentage of embryos with wild-type pancreas following injection of PesATG MO into a p53zdf1/zdf1;ptf1a:eGFPjh1;ins:mCherryjh2 background. (C) The gross appearance of PesATG MO-injected embryos at 72 hpf includes a small head and eye but normal body axis. (D) Microarray quantification of pes transcript levels indicates an increase in pes expression in SbdsATG MO-injected p53zdf1/zdf1 embryos. (E) Increased abundance of pes transcripts in SbdsATG MO-injected p53zdf1/zdf1 embryos is confirmed by in situ hybridization. Error bars indicate s.e.m.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP tp53zdf1/zdf1; jh1Tg; jh2Tg control Protruding-mouth central nervous system IFL
Protruding-mouth pancreas IFL
Protruding-mouth pancreas somatic stem cell IFL
tp53zdf1/zdf1; jh1Tg; jh2Tg + MO1-pes standard conditions Protruding-mouth central nervous system IFL
mCherry tp53zdf1/zdf1; jh1Tg; jh2Tg control Protruding-mouth endocrine pancreas IFL
tp53zdf1/zdf1; jh1Tg; jh2Tg + MO1-pes standard conditions Protruding-mouth endocrine pancreas IFL
pes tp53zdf1/zdf1 control Prim-5 eye ISH
Prim-5 midbrain ISH
Long-pec endoderm ISH
Long-pec pancreas ISH
tp53zdf1/zdf1 + MO4-sbds standard conditions Prim-5 eye ISH
Prim-5 midbrain ISH
Prim-5 somite ISH
Long-pec endoderm ISH
Long-pec head ISH
Long-pec somite ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
tp53zdf1/zdf1 + MO4-sbds standard conditions Prim-5 exocrine pancreas decreased size, abnormal
Prim-5 exocrine pancreas development disrupted, abnormal
Long-pec exocrine pancreas decreased size, abnormal
Long-pec exocrine pancreas development disrupted, abnormal
tp53zdf1/zdf1; jh1Tg; jh2Tg + MO1-pes standard conditions Protruding-mouth endocrine pancreas position, normal
Protruding-mouth exocrine pancreas decreased size, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth eye decreased size, abnormal
Protruding-mouth head decreased size, abnormal
Protruding-mouth head opacity, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development