ZFIN ID: ZDB-FIG-110128-29
Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. Proceedings of the National Academy of Sciences of the United States of America   107(6):2521-2526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Shield
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Shield

Fig. 4 Setdb2 restricts dorsal organizer formation and regulates left–right asymmetry through suppressing Fgf8 activity. (AF) WISH analyses of organizer markers flh (A and B), gsc (C and D), and chd (E and F) in the 5-MM control and setdb2 MO2-injected embryos at 6 hpf. Double-headed arrows denote territories of dorsal organizer. Embryos are animal views in the Upper panels, dorsal views in the Bottom panels. (GI) WISH analyses of fgf8 expression (G and H) in the 5-MM control and setdb2 MO2-injected embryos at 6 hpf and effects of fgf8 overexpression on the dorsal organizer development as revealed by flh expression (I). Embryos are animal views in panels G and H (Upper) and I and dorsal views in panels G and H (Bottom). (JM) WISH analyses of flh in the 6-hpf embryos injected with 5-MM control (J), setdb2 MO2 (K), setdb2 MO2 plus dominant-negative fibroblast growth factor receptor (dnfgfr) mRNAs (L), and setdb2 MO2 plus fgf8 morpholino oligonucleotides (M). (N) Statistic analyses of heart laterality marker cmlc2 and asymmetric gene southpaw in 6-hpf embryos injected with setdb2 MO2 and setdb2 MO2 plus fgf8 morpholino. Embryos are animal views in the Upper Panels and dorsal views in the Lower Panels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
chrd WT control Shield shield ISH
WT + MO2-setdb2 standard conditions Shield shield ISH
fgf8a WT control Shield germ ring ISH
Shield shield ISH
WT + MO2-setdb2 standard conditions Shield germ ring ISH
Shield shield ISH
gsc WT control Shield shield ISH
WT + MO2-setdb2 standard conditions Shield shield ISH
noto WT control Shield shield ISH
WT + MO2-setdb2 standard conditions Shield shield ISH
WT + MO2-setdb2 + MO3-fgf8a standard conditions Shield shield ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-setdb2 standard conditions Shield determination of left/right symmetry disrupted, abnormal
Shield shield distended, abnormal
WT + MO2-setdb2 + MO3-fgf8a standard conditions Shield determination of left/right symmetry occurrence, normal
Shield shield size, normal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA