ZFIN ID: ZDB-FIG-110128-32
Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. Proc. Natl. Acad. Sci. USA   107(6):2521-2526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Protruding-mouth

Fig. S4 Organ laterality is randomized in setdb2-knockdown embryos with MO1. (A–D) Whole-mount mRNA in situ hybridization of visceral and diencephalic laterality in the setdb2 MO1-injected embryos at the indicated developmental stages with probes cmlc2 (A), lefty1 (B), cebpα (C, arrows), and insulin (D). (E) Determination of pancreatic laterality in 72-hpf Tg(insulin:EGFP) transgenic embryos injected with setdb2 MO1. Dashed lines denote the embryonic midline. LV, liver; G, gut. All embryos are dorsal views with head to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cebpa WT + MO1-setdb2 standard conditions Protruding-mouth gut ISH
Protruding-mouth liver ISH
EGFP rj29Tg + MO1-setdb2 standard conditions Protruding-mouth pancreas IFL
ins WT + MO1-setdb2 standard conditions Protruding-mouth pancreas ISH
lft1 WT + MO1-setdb2 standard conditions 20-25 somites diencephalon ISH
myl7 WT + MO1-setdb2 standard conditions Prim-15 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-setdb2 standard conditions 20-25 somites determination of left/right asymmetry in diencephalon disrupted, abnormal
WT + MO1-setdb2 standard conditions 20-25 somites diencephalon structure, abnormal
WT + MO1-setdb2 standard conditions Prim-15 determination of heart left/right asymmetry disrupted, abnormal
WT + MO1-setdb2 standard conditions Prim-15 heart tube centered, abnormal
WT + MO1-setdb2 standard conditions Prim-15 heart tube mislocalised radially, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth determination of digestive tract left/right asymmetry disrupted, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth determination of liver left/right asymmetry disrupted, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth determination of pancreatic left/right asymmetry disrupted, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth gut centered, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth gut mislocalised radially, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth liver centered, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth liver mislocalised radially, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth pancreas centered, abnormal
WT + MO1-setdb2 standard conditions Protruding-mouth pancreas mislocalised radially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA