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ZIRC
ZFIN ID: ZDB-FIG-110128-33
Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. Proceedings of the National Academy of Sciences of the United States of America   107(6):2521-2526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to Prim-5
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Bud to Prim-5

Fig. S5 Defective midline development in setdb2-knockdown embryos. (A–L) Whole-mount in situ hybridization analyses of midline markers oep (A–D), shh (E–H), and ntl (I–L) in the 5-MM control and setdb2 MO2-injected embryos at the indicated developmental stages. Embryos are dorsal views with head to the top in panels A, B, E, F, I, and J and to the left in panels C and D. Lateral views with head to the left in panels G, H, K, and L.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
shha WT control Bud notochord ISH
Prim-5 notochord ISH
WT + MO2-setdb2 standard conditions Bud notochord ISH
Prim-5 notochord ISH
tbxta WT control Bud forerunner cell group ISH
Bud notochord ISH
Prim-5 notochord ISH
Prim-5 tail bud ISH
WT + MO2-setdb2 standard conditions Bud forerunner cell group ISH
Bud notochord ISH
Prim-5 notochord ISH
Prim-5 tail bud ISH
tdgf1 WT control Bud axis ISH
Bud neural plate dorsal region ISH
14-19 somites notochord ISH
WT + MO2-setdb2 standard conditions Bud axis ISH
Bud neural plate dorsal region ISH
14-19 somites notochord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-setdb2 standard conditions Bud axis decreased length, abnormal
Bud axis increased width, abnormal
14-19 somites notochord undulate, abnormal
Prim-5 notochord undulate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA