ZFIN ID: ZDB-FIG-110128-28
Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. Proceedings of the National Academy of Sciences of the United States of America   107(6):2521-2526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 90%-epiboly to 26+ somites
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 5-9 somites to 26+ somites

Fig. 3 The expression of asymmetric genes is randomized and cilia development in the Kupffer’s vesicle is disrupted in setdb2-knockdown embryos. (AI) Whole-mount mRNA in situ hybridization and statistical analyses of the expression of asymmetric genes southpaw (AC), pitx2 (DF, arrows), and lefty2 (GI) in the 5-MM control and setdb2 MO2-injected embryos at the indicated developmental stages. L, Left side; R, Right side; C/B, central or bilateral; A, absent. All embryos are dorsal views with head to the top. (J–M) WISH analyses of charon (J and K, arrows) and lrdr1 expression (L and M, arrows) in the 5-MM control and setdb2 MO2-injected embryos. (NQ) Morphology of Kupffer’s vesicle (N and O, arrows) and confocol analyses of ciliary development (P and Q, green) as revealed by immunostaining with anti-acetylated tubulin antibody. The numbers in P and Q denote the mean ± SD. Embryos are ventral views in J, K, N, and O and lateral views in L and M.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dand5 WT control 5-9 somites Kupffer's vesicle ISH
WT + MO2-setdb2 standard conditions 5-9 somites Kupffer's vesicle ISH
dnah9 WT control 90%-epiboly forerunner cell group ISH
WT + MO2-setdb2 standard conditions 90%-epiboly forerunner cell group ISH
lft2 WT control 26+ somites primitive heart tube ISH
WT + MO2-setdb2 standard conditions 26+ somites primitive heart tube ISH
pitx2 WT control 20-25 somites lateral plate mesoderm left side ISH
WT + MO2-setdb2 standard conditions 20-25 somites lateral plate mesoderm left side ISH
20-25 somites lateral plate mesoderm right side ISH
spaw WT control 20-25 somites lateral plate mesoderm left side ISH
WT + MO2-setdb2 standard conditions 20-25 somites lateral plate mesoderm left side ISH
20-25 somites lateral plate mesoderm right side ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT control 5-9 somites Kupffer's vesicle cilium
IHC WT + MO2-setdb2 standard conditions 5-9 somites Kupffer's vesicle cilium
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-setdb2 standard conditions 5-9 somites Kupffer's vesicle morphology, normal
5-9 somites Kupffer's vesicle cilium decreased amount, abnormal
20-25 somites determination of left/right asymmetry in lateral mesoderm disrupted, abnormal
20-25 somites lateral plate mesoderm structure, abnormal
26+ somites determination of heart left/right asymmetry disrupted, abnormal
26+ somites primitive heart tube centered, abnormal
26+ somites primitive heart tube mislocalised radially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proceedings of the National Academy of Sciences of the United States of America for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA