ZFIN ID: ZDB-FIG-110128-27
Xu et al., 2010 - Setdb2 restricts dorsal organizer territory and regulates left-right asymmetry through suppressing fgf8 activity. Proc. Natl. Acad. Sci. USA   107(6):2521-2526 Full text @ Proc. Natl. Acad. Sci. USA
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: 20-25 somites to Protruding-mouth

Fig. 2 Organ laterality is randomized in setdb2-knockdown embryos. (A–L) Whole-mount mRNA in situ hybridization and statistical analyses of visceral and diencephalic laterality in the 5-MM control and setdb2 MO2-injected embryos at the indicated developmental stages with probes cmlc2 (A–C), lefty1 (DF), cebpα (GI, arrows), and insulin (JL). (MO) Determination of pancreatic laterality in 72-hpf Tg(insulin:EGFP) transgenic embryos injected with 5-MM control and setdb2 MO2. Dashed lines denote the embryonic midline. LV, liver; G, gut; L, Left side; R, Right side; C/B, central or bilateral; A, absent. All embryos are dorsal views with head to the top.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cebpa WT control Long-pec gut ISH
Long-pec liver ISH
WT + MO2-setdb2 standard conditions Long-pec gut ISH
Long-pec liver ISH
EGFP rj29Tg control Protruding-mouth pancreas IFL
rj29Tg + MO2-setdb2 standard conditions Protruding-mouth pancreas IFL
ins WT control Protruding-mouth pancreas ISH
WT + MO2-setdb2 standard conditions Protruding-mouth pancreas ISH
lft1 WT control 20-25 somites diencephalon ISH
WT + MO2-setdb2 standard conditions 20-25 somites diencephalon ISH
myl7 WT control Prim-15 heart tube ISH
WT + MO2-setdb2 standard conditions Prim-15 heart tube ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-setdb2 standard conditions 20-25 somites determination of left/right asymmetry in diencephalon disrupted, abnormal
20-25 somites diencephalon structure, abnormal
Prim-15 determination of heart left/right asymmetry disrupted, abnormal
Prim-15 heart tube centered, abnormal
Prim-15 heart tube mislocalised radially, abnormal
Long-pec determination of digestive tract left/right asymmetry disrupted, abnormal
Long-pec determination of liver left/right asymmetry disrupted, abnormal
Long-pec gut centered, abnormal
Long-pec gut mislocalised radially, abnormal
Long-pec liver centered, abnormal
Long-pec liver mislocalised radially, abnormal
Protruding-mouth determination of pancreatic left/right asymmetry disrupted, abnormal
Protruding-mouth pancreas centered, abnormal
Protruding-mouth pancreas mislocalised radially, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Proc. Natl. Acad. Sci. USA for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Proc. Natl. Acad. Sci. USA