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ZFIN ID: ZDB-FIG-110121-10
Choudhry et al., 2011 - Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis. Developmental Biology   349(2):483-493 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15

Fig. 4 alcama is required for edn1-dependent gene expression. Ventral views of larvae at 30 hpf (A–C), and lateral views at 30 hpf (D–L) and at 48 hpf (M–O) respectively. ISH for hand2 (A–C), dlx3b (D–F), dlx5a (G–I), dlx6a (J–L), and bapx1 (M–O) in control and alcama morphants and in edn1-/- mutants. hand2, dlx3b, dlx5a and dlx6a expression is strongly reduced in the first and branchial arches and moderately reduced in the second arch in alcama morphants. Their expression is strongly reduced in all arches in the edn1-/- mutants. bapx1 expression in the first arch intermediate domain is moderately reduced in alcama morphants and strongly reduced in edn1-/- mutants. a1 and a2 label pharyngeal arches 1 and 2. Dotted lines highlight pharyngeal pouches 1 and 2. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT control Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 3 ISH
WT + MO4-alcama standard conditions Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch 2 ISH
dlx5a WT control Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
Prim-15 pharyngeal arch 3 ISH
Prim-15 pharyngeal arch 4 ISH
WT + MO4-alcama standard conditions Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch 2 ISH
dlx6a WT control Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 3 ISH
WT + MO4-alcama standard conditions Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch 2 ISH
hand2 WT control Prim-15 heart tube ISH
Prim-15 pharyngeal arch 1 ISH
Prim-15 pharyngeal arch 2 ISH
Prim-15 pharyngeal arch 3 ISH
WT + MO4-alcama standard conditions Prim-15 heart tube ISH
Prim-15 pharyngeal arch 2 ISH
edn1tf216b/tf216b control Prim-15 heart tube ISH
nkx3-2 WT control Prim-15 pharyngeal arch 1 joint ISH
WT + MO4-alcama standard conditions Prim-15 pharyngeal arch 1 ISH
edn1tf216b/tf216b standard conditions Prim-15 pharyngeal arch 1 ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.