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Fig. 4

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ZDB-IMAGE-110121-5
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Figures for Choudhry et al., 2011
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Fig. 4 alcama is required for edn1-dependent gene expression. Ventral views of larvae at 30 hpf (A–C), and lateral views at 30 hpf (D–L) and at 48 hpf (M–O) respectively. ISH for hand2 (A–C), dlx3b (D–F), dlx5a (G–I), dlx6a (J–L), and bapx1 (M–O) in control and alcama morphants and in edn1-/- mutants. hand2, dlx3b, dlx5a and dlx6a expression is strongly reduced in the first and branchial arches and moderately reduced in the second arch in alcama morphants. Their expression is strongly reduced in all arches in the edn1-/- mutants. bapx1 expression in the first arch intermediate domain is moderately reduced in alcama morphants and strongly reduced in edn1-/- mutants. a1 and a2 label pharyngeal arches 1 and 2. Dotted lines highlight pharyngeal pouches 1 and 2. Scale bars: 50 μm.

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Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.