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ZFIN ID: ZDB-FIG-110121-8
Choudhry et al., 2011 - Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis. Developmental Biology   349(2):483-493 Full text @ Dev. Biol.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Day 5

Fig. 2 alcama morphants have cartilage defects similar to the edn1 class of mutants. Flatmounts of mandibular and hyoid cartilage from 5 dpf Alcian Blue-stained larvae (A, C, E, G); corresponding schematics (B, D, F, H). The joint between Meckel′s and palatoquadrate is fused in alcama morphants and furinA-/- and edn1-/- mutants. Similarly, the interhyal is absent in alcama morphants and furinA-/- mutants leading to a fusion of the ceratohyal and hyosymplectic cartilages. alcama morphants and furinA-/- mutants have misshapen Meckel′s cartilage and ceratohyal, but edn1-/- mutants have the most severe defect with a lack of ceratohyal and severely misshapen Meckel′s cartilage. DV joint regions are indicated with arrows in A and B. Fusions at joints are indicated with asterisks in (C)–(H). Cartilages are labeled as followed: pq (palatoquadrate), mc (Meckel′s cartilage), hm (hyomandibula), ch (ceratohyal), sy (symplectic), and ih (interhyal). Scale bar: 50 μm.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO4-alcama standard conditions Day 5 ceratohyal cartilage fused with hyosymplectic cartilage, abnormal
Day 5 interhyal cartilage absent, abnormal
Day 5 Meckel's cartilage bent, abnormal
Day 5 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
edn1tf216b/tf216b standard conditions Day 5 Meckel's cartilage bent, abnormal
Day 5 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
Day 5 Meckel's cartilage protruding, abnormal
Day 5 palatoquadrate cartilage fused with hyosymplectic cartilage, abnormal
Day 5 palatoquadrate cartilage increased length, abnormal
Day 5 symplectic absent, abnormal
furinatd204e/td204e standard conditions Day 5 ceratohyal cartilage fused with hyosymplectic cartilage, abnormal
Day 5 Meckel's cartilage fused with palatoquadrate cartilage, abnormal
Day 5 palatoquadrate cartilage increased length, abnormal
Day 5 symplectic in contact with Meckel's cartilage, abnormal
Day 5 symplectic increased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 349(2), Choudhry, P., Joshi, D., Funke, B., and Trede, N., Alcama mediates Edn1 signaling during zebrafish cartilage morphogenesis, 483-493, Copyright (2011) with permission from Elsevier. Full text @ Dev. Biol.