ZFIN ID: ZDB-FIG-101021-21
Kwon et al., 2010 - Identification of early requirements for preplacodal ectoderm and sensory organ development. PLoS Genetics   6(9):pii: e1001133 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Conditions:
Anatomical Terms:
Stage Range: 75%-epiboly to 1-4 somites
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: 1-4 somites

Fig. 3 Stage-dependent requirements for Bmp.

(A–E, G–L) Analysis of indicated gene expression patterns in control embryos and embryos treated with 100 µM dorsomorphin (DM) at 5 hpf or 7 hpf. Lateral views with dorsal to the right and anterior up. Expression of six4.1, eya1 and dlx3b (A–C) in PPE, krox20 in hindbrain(D) and p63 in epidermal ectoderm (E). Expression of competence factor genes tfap2a, tfap2c, foxi1 and gata3 (G–J). Reporters of Bmp-signaling, Phospho-Smad1/5/8 antibody staining (K) and sizzled in situ hybridization (L). Note the complete loss of Bmp signaling by 100 µM DM-treatment either at 5 hpf or 7 hpf. (F) Lateral views of live embryos at 30 hpf. Embryos treated with DM at 7 hpf show a partially dorsalized C3 phenotype [33]. (M, N) Tg(hs:chd) and/or Tg(hs:dnBmpr) embryos heat-shocked and treated with 100 µM DM at 7.5 hpf. eya1 expression (M) and C3 phenotypes (N) are comparable to embryos treated with 100 µM DM alone.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT control 1-4 somites ectoderm ISH
WT chemical treatment: pharmaceutical 1-4 somites ectoderm ISH
egr2b WT control 1-4 somites hindbrain neural plate ISH
WT chemical treatment: pharmaceutical 1-4 somites hindbrain neural plate ISH
eya1 WT control 1-4 somites ectoderm ISH
WT chemical treatment: pharmaceutical 1-4 somites ectoderm ISH
p02Tg/p02Tg heat shock, chemical treatment: pharmaceutical 1-4 somites ectoderm ISH
p02Tg/p02Tg; w30Tg/+ heat shock 1-4 somites ectoderm ISH
p02Tg/p02Tg; w30Tg/+ heat shock, chemical treatment: pharmaceutical 1-4 somites ectoderm ISH
foxi1 WT control 75%-epiboly ectoderm ISH
WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
gata3 WT control 75%-epiboly ectoderm ISH
WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
six4b WT control 1-4 somites ectoderm ISH
WT chemical treatment: pharmaceutical 1-4 somites ectoderm ISH
tfap2a WT control 75%-epiboly ectoderm ISH
WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
tfap2c WT control 75%-epiboly ectoderm ISH
WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
tp63 WT control Bud ectoderm ISH
WT chemical treatment: pharmaceutical Bud ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
p02Tg/p02Tg heat shock, chemical treatment: pharmaceutical 1-4 somites whole organism wholly dorsalized, abnormal
p02Tg/p02Tg; w30Tg/+ heat shock 1-4 somites whole organism wholly dorsalized, abnormal
p02Tg/p02Tg; w30Tg/+ heat shock, chemical treatment: pharmaceutical 1-4 somites whole organism wholly dorsalized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.