ZFIN ID: ZDB-FIG-101021-20
Kwon et al., 2010 - Identification of early requirements for preplacodal ectoderm and sensory organ development. PLoS Genetics   6(9):pii: e1001133 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage Range: 75%-epiboly to 1-4 somites
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: 1-4 somites

Fig. 2 Distinct responses of neural crest and preplacodal ectoderm to graded impairment of Bmp.

(A) Lateral views of foxd3 expression at 11 hpf with anterior up and dorsal to the right. Embryos were treated with indicated concentrations of DM added at 4 hpf. Where indicated the DM concentration was increased to 200 µM (complete Bmp-inhibition) at 5 hpf, 6 hpf or 7 hpf. (B) Lateral views of six4.1 expression at 11 hpf in embryos treated with indicated concentrations of DM beginning at 4 hpf. Treatment with 25 µM DM yields two discrete responses, one in which six4.1 remains confined to two bilateral stripes flanking the neural plate and the other in which six4.1 expression is lost. (C) Lateral views showing expression of preplacodal competence factors tfap2a, tfap2c, foxi1 and gata3 in embryos were treated with 50 µM DM beginning at 4 hpf. Note that tfap2a/c remain broadly expressed in ventral ectoderm whereas foxi1 and gata3 are nearly eliminated.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
foxd3 WT control 1-4 somites neural crest ISH
foxi1 WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
gata3 WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
six4b WT chemical treatment: pharmaceutical 1-4 somites ectoderm ISH
tfap2a WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
tfap2c WT chemical treatment: pharmaceutical 75%-epiboly ectoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: pharmaceutical 1-4 somites cranial neural crest wholly ventralized, abnormal
WT chemical treatment: pharmaceutical 1-4 somites neural crest absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.