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ZFIN ID: ZDB-FIG-090224-32
Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Development (Cambridge, England)   136(5):781-789 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites

Fig. 5 Spinal progenitors lacking Tcf3 precociously express neuronal markers. (A,B) Cross-sections of tubb5 in situ hybridization at 18 hpf show that tcf3 morphants have ectopic tubb5-positive cells in the medial progenitor domain (arrows). (C,D) Double labeling for HuC/D (green) and BrdU (red) at 24 hpf shows BrdU-positive Hu-positive cells (arrows) in tcf3 morphants, some of which are in the medial domain. (E-N) Lateral whole-mount views of postmitotic neuronal markers at 18 hpf. (E-H) lhx1a and pax2a, which mark multiple classes of spinal interneurons, are drastically reduced in tcf3 morphants. (I-N) evx1+ interneurons, en1b+ interneurons and isl1+ primary motoneurons (asterisks) are also reduced in tcf3 morphants.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
en1b AB standard conditions 14-19 somites spinal cord interneuron ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord interneuron ISH
evx1 AB standard conditions 14-19 somites spinal cord interneuron ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord interneuron ISH
isl1 AB standard conditions 14-19 somites spinal cord neural tube motor neuron ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord neural tube motor neuron ISH
lhx1a AB standard conditions 14-19 somites spinal cord interneuron ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord interneuron ISH
pax2a AB standard conditions 14-19 somites spinal cord interneuron ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord interneuron ISH
tubb5 WT standard conditions 14-19 somites spinal cord neural tube ISH
WT + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord neural tube ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development