ZFIN ID: ZDB-FIG-090224-30
Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Development (Cambridge, England)   136(5):781-789 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites

Fig. 3 dbx1a expression is absent in tcf3 morphants. (A,A′) Whole-mount lateral and cross-section views of 18 hpf wild-type embryos hybridized for dbx1a mRNA. (B,B′) tcf3 morphant embryos hybridized for dbx1a, showing loss of expression throughout intermediate spinal progenitors. Residual expression is likely to be in postmitotic neurons. (C,D) Double in situ hybridization for dbx1a (red) and tcf3 (blue), showing overlap between the two genes. (E,F) Both wild-type (E) and tcf3 morphants (F) have a similar gap (red lines) between the pax3 (dorsal) and nkx6.1 (ventral) expression domains, suggesting that a general dorsoventral shift in patterning has not occurred.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a AB standard conditions 14-19 somites spinal cord neural tube neuroblast (sensu Vertebrata) ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord neural tube neuroblast (sensu Vertebrata) ISH
nkx6.1 AB standard conditions 14-19 somites spinal cord neural tube ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord neural tube ISH
pax3a AB standard conditions 14-19 somites spinal cord neural tube ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions 14-19 somites spinal cord neural tube ISH
tcf7l1a AB standard conditions 14-19 somites spinal cord neural tube RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development