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ZIRC
ZFIN ID: ZDB-FIG-090224-33
Gribble et al., 2009 - Tcf3 inhibits spinal cord neurogenesis by regulating sox4a expression. Development (Cambridge, England)   136(5):781-789 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 6 sox4a is repressed by Tcf3 in spinal progenitors. (A,B) sox4a is weakly expressed in the spinal cord of wild-type embryos at 24 hpf and is ectopically expressed throughout the intermediate and ventral spinal cord of tcf3 morphants. (C) Overexpression of sox4a mRNA leads to ectopic HuC/D BrdU double-positive cells in medial progenitors (arrows). (D,E) sox3 is expressed in intermediate and ventral spinal progenitors of wild-type embryos at 24 hpf, and is drastically reduced in tcf3 morphants. (F) sox11a is expressed in both spinal progenitors and postmitotic neurons at 24 hpf. (G,H) sox4a overexpression does not affect the expression of dbx1a in spinal progenitors.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a AB standard conditions Prim-5 spinal cord neuroblast (sensu Vertebrata) ISH
sox3 AB standard conditions Prim-5 spinal cord ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 spinal cord ISH
sox4a AB standard conditions Prim-5 spinal cord ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 spinal cord ISH
sox11a AB standard conditions Prim-5 spinal cord neuroblast (sensu Vertebrata) ISH
Prim-5 spinal cord neuron ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 generation of neurons disrupted, abnormal
Prim-5 spinal cord neuroblast (sensu Vertebrata) cellular quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development