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ZFIN ID: ZDB-FIG-090220-59
Devine et al., 2009 - A dynamic Gli code interprets Hh signals to regulate induction, patterning, and endocrine cell specification in the zebrafish pituitary. Developmental Biology   326(1):143-154 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Long-pec

Fig. 4

Gli function in endocrine cell determination. (A–D) Normal expression of prl, gh, tsh-β and pomc in the adenohypophysis at 48 hpf (ventral view, anterior left). The adenohypophysis is outlined. (E–H) Loss of Gli1 function either by gli1 MO injection or in dtr(gli1-/-) mutants (not shown) reduced prl (E) and anterior pomc (H) expressing cells and eliminated gh (F) expressing cells. tsh-β (G) and posterior pomc cells (H) were relatively unaffected by loss of Gli1 function. (I–L) In contrast, loss of Gli2b function expanded prl (I) and tsh-β (K) populations, slightly reduced anterior pomc (L) and gh (J) expressing cells, and had little effect on posterior pomc cells (L). Hypothalamic (hy) pomc populations appeared to expand in response to reduced Gli2b function (L). Injection of gli2a MOs also expanded prl cells posteriorly (I, inset) in 40% of embryos examined. (M–P) Simultaneous loss of Gli2a and Gli2b function further expanded prl populations (M), reduced anterior pomc cells (P) and had little effect on gh (N), tsh-β (O) and posterior pomc (P) expressing cells. (Q–T) Loss of Gli1 in combination with loss of Gli2a and Gli2b eliminated the expansion of prl cells seen following Gli2b MO injection (Q), further reduced gh (R) and anterior pomc cells (T), and slightly expanded tsh-β cell numbers (S). (U) Graph showing cell numbers across all Gli loss-of-function experiments, represented as percentage of wild type cell numbers. Gray shading shows the results following injection of gli2 (2a and/or 2b) MOs in a wild type background. Black shading shows the results obtained following injection of gli2 (2a and/or 2b) MOs in a gli1 loss-of-function background. Scale bar: A–T; 25 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
gh1 WT control Long-pec adenohypophyseal placode ISH
Long-pec somatotropin secreting cell ISH
WT + MO1-gli1 standard conditions Long-pec Not Detected adenohypophyseal placode ISH
WT + MO1-gli1 + MO1-gli2a + MO3-gli2b standard conditions Long-pec Not Detected adenohypophyseal placode ISH
WT + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec somatotropin secreting cell ISH
WT + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec somatotropin secreting cell ISH
pomca WT control Long-pec adenohypophyseal placode ISH
Long-pec hypothalamus ISH
Long-pec melanocyte stimulating hormone secreting cell ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli1 standard conditions Long-pec adenohypophyseal placode ISH
Long-pec hypothalamus ISH
Long-pec melanocyte stimulating hormone secreting cell ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli1 + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec hypothalamus ISH
Long-pec melanocyte stimulating hormone secreting cell ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec hypothalamus ISH
Long-pec melanocyte stimulating hormone secreting cell ISH
Long-pec peptide hormone secreting cell ISH
WT + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec hypothalamus ISH
Long-pec melanocyte stimulating hormone secreting cell ISH
Long-pec peptide hormone secreting cell ISH
prl WT control Long-pec adenohypophyseal placode ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli1 standard conditions Long-pec adenohypophyseal placode ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli1 + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli2a standard conditions Long-pec adenohypophyseal placode ISH
Long-pec peptide hormone secreting cell ISH
WT + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec peptide hormone secreting cell ISH
WT + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec peptide hormone secreting cell ISH
tshba WT control Long-pec adenohypophyseal placode ISH
Long-pec thyroid stimulating hormone secreting cell ISH
WT + MO1-gli1 standard conditions Long-pec adenohypophyseal placode ISH
Long-pec thyroid stimulating hormone secreting cell ISH
WT + MO1-gli1 + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec thyroid stimulating hormone secreting cell ISH
WT + MO1-gli2a standard conditions Long-pec adenohypophyseal placode ISH
Long-pec thyroid stimulating hormone secreting cell ISH
WT + MO1-gli2a + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec thyroid stimulating hormone secreting cell ISH
WT + MO3-gli2b standard conditions Long-pec adenohypophyseal placode ISH
Long-pec thyroid stimulating hormone secreting cell ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli1 standard conditions Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
WT + MO1-gli1 + MO1-gli2a + MO3-gli2b standard conditions Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
WT + MO1-gli2a standard conditions Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec prolactin secreting cell normal amount, normal
Long-pec somatotropin secreting cell decreased amount, abnormal
WT + MO1-gli2a + MO3-gli2b standard conditions Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec melanocyte stimulating hormone secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell increased amount, abnormal
Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
Long-pec thyroid stimulating hormone secreting cell normal amount, normal
WT + MO3-gli2b standard conditions Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec melanocyte stimulating hormone secreting cell normal amount, normal
Long-pec prolactin secreting cell increased amount, abnormal
Long-pec prolactin secreting cell differentiation process quality, abnormal
Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
Long-pec thyroid stimulating hormone secreting cell increased amount, abnormal
gli1ts269/ts269 control Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec prolactin secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell differentiation process quality, abnormal
Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
gli1ts269/ts269 + MO1-gli2a standard conditions Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec prolactin secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell differentiation process quality, abnormal
Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
Long-pec thyroid stimulating hormone secreting cell decreased amount, abnormal
Long-pec thyroid-stimulating hormone-secreting cell differentiation process quality, abnormal
gli1ts269/ts269 + MO1-gli2a + MO3-gli2b standard conditions Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec melanocyte stimulating hormone secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell differentiation process quality, abnormal
Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
Long-pec thyroid stimulating hormone secreting cell normal amount, normal
gli1ts269/ts269 + MO3-gli2b standard conditions Long-pec adrenocorticotropic hormone secreting cell decreased amount, abnormal
Long-pec corticotropin hormone secreting cell differentiation process quality, abnormal
Long-pec melanocyte stimulating hormone secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell decreased amount, abnormal
Long-pec prolactin secreting cell differentiation process quality, abnormal
Long-pec somatotropin secreting cell decreased amount, abnormal
Long-pec somatotropin secreting cell differentiation process quality, abnormal
Long-pec thyroid stimulating hormone secreting cell normal amount, normal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 326(1), Devine, C.A., Sbrogna, J.L., Guner, B., Osgood, M., Shen, M.C., and Karlstrom, R.O., A dynamic Gli code interprets Hh signals to regulate induction, patterning, and endocrine cell specification in the zebrafish pituitary, 143-154, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.