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ZFIN ID: ZDB-FIG-090220-58
Devine et al., 2009 - A dynamic Gli code interprets Hh signals to regulate induction, patterning, and endocrine cell specification in the zebrafish pituitary. Developmental Biology   326(1):143-154 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-15

Fig. 3 Gli1, Gli2a, and Gli2b contribute to the Hh response in the pituitary. (A) At 30 hpf nkx2.2a is expressed in the PD (arrowhead) but not in the PI (arrow). lim3 (inset) is expressed in both the PD and PI. (B) In dtr(gli1) mutants, nkx2.2a expression is reduced in the PD (arrowhead) and lim3 expression is reduced, indicating a smaller placode (inset). (C) Injecting gli2a morpholinos (MOs) into wildtype embryos led to a posterior expansion of nkx2.2a and lim3 expression (arrows). (D) Injecting gli2a MOs into dtr(gli1) mutants eliminated nkx2.2a expression in the adenohypophysis, but did not further reduce lim3 expression (inset). (E) Injecting gli2b MOs into wildtype embryos led to a posterior expansion of nkx2.2a and lim3 expression (arrows). (F) gli2b MO injections into dtr(gli1) mutants eliminated nkx2.2a expression in the placode, but did not further reduce lim3 expression (inset). (G) Co-injection of gli2a and gli2b MOs led to posterior expansion of nkx2.2a and lim3 expression (arrows). (H) Co-injection of gli2a and gli2b MOs eliminated nkx2.2a expression and reduced lim3 expression. (I) Dominant repressor forms of Gli2a encoded by the yot locus eliminate nkx2.2a expression in the PD. (J) gli2a MO injections into yot(gli2DR) rescued nkx2.2a expression (arrowhead), with expression resembling that seen in dtr(gli1) mutants (compare to panel B). (A–J) Lateral views of the ventral forebrain, anterior to the left, eyes removed. Scale bar: A–J; 30 μm, insets in panels A–H; 40 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lhx3 WT standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
WT + MO1-gli2a standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
WT + MO1-gli2a + MO3-gli2b standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
WT + MO3-gli2b standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
gli1ts269/ts269 standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
gli1ts269/ts269 + MO1-gli2a standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
gli1ts269/ts269 + MO1-gli2a + MO3-gli2b standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
gli1ts269/ts269 + MO3-gli2b standard conditions Prim-15 pars anterior ISH
Prim-15 pars intermedia ISH
nkx2.2a WT standard conditions Prim-15 pars anterior ISH
WT + MO1-gli2a standard conditions Prim-15 pars anterior ISH
WT + MO1-gli2a + MO3-gli2b standard conditions Prim-15 pars anterior ISH
WT + MO3-gli2b standard conditions Prim-15 pars anterior ISH
gli1ts269/ts269 standard conditions Prim-15 pars anterior ISH
gli1ts269/ts269 + MO1-gli2a standard conditions Prim-15 Not Detected adenohypophyseal placode ISH
gli1ts269/ts269 + MO1-gli2a + MO3-gli2b standard conditions Prim-15 Not Detected adenohypophyseal placode ISH
gli1ts269/ts269 + MO3-gli2b standard conditions Prim-15 Not Detected adenohypophyseal placode ISH
gli2aty17a/ty17a standard conditions Prim-15 Not Detected adenohypophyseal placode ISH
gli2aty17a/ty17a + MO1-gli2a standard conditions Prim-15 pars anterior ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli2a standard conditions Prim-15 adenohypophysis wholly anterioralized, abnormal
Prim-15 adenohypophysis morphogenesis process quality, abnormal
WT + MO1-gli2a + MO3-gli2b standard conditions Prim-15 adenohypophysis wholly anterioralized, abnormal
Prim-15 adenohypophysis morphogenesis process quality, abnormal
WT + MO3-gli2b standard conditions Prim-15 adenohypophysis wholly anterioralized, abnormal
Prim-15 adenohypophysis morphogenesis process quality, abnormal
gli1ts269/ts269 standard conditions Prim-15 adenohypophyseal placode decreased size, abnormal
Prim-15 adenohypophysis morphogenesis process quality, abnormal
gli1ts269/ts269 + MO1-gli2a standard conditions Prim-15 adenohypophysis morphogenesis process quality, abnormal
Prim-15 pars anterior physical object quality, abnormal
gli1ts269/ts269 + MO3-gli2b standard conditions Prim-15 adenohypophysis morphogenesis process quality, abnormal
Prim-15 pars anterior physical object quality, abnormal
gli2aty17a/ty17a standard conditions Prim-15 adenohypophysis morphogenesis process quality, abnormal
Prim-15 pars anterior physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 326(1), Devine, C.A., Sbrogna, J.L., Guner, B., Osgood, M., Shen, M.C., and Karlstrom, R.O., A dynamic Gli code interprets Hh signals to regulate induction, patterning, and endocrine cell specification in the zebrafish pituitary, 143-154, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.