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ZFIN ID: ZDB-FIG-070307-21
Nyholm et al., 2007 - The zebrafish zic2a-zic5 gene pair acts downstream of canonical Wnt signaling to control cell proliferation in the developing tectum. Development (Cambridge, England)   134(4):735-746 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Shield to Prim-5

Fig. 1 Zic2a and zic5 are linked and co-expressed in presumptive dorsal brain. (A) Genomic arrangement of zic2a and zic5. Coding regions are shown as gray boxes, non-coding transcribed regions as white boxes, and introns as lines. (B-G) Embryos stained by ISH for zic2a or zic5 (purple), and dlx3 (orange). (B) At midgastrula stages, zic2a is expressed throughout neurectoderm, whereas dlx3 marks the adjacent non-neural ectoderm. (C) By late gastrula stages, zic2a is restricted to the lateral neural plate and forms a sharp border with dlx3. (D,E) During somitogenesis, zic2a and zic5 are similarly expressed in the dorsal neural tube, ventral diencephalon and optic stalks. (F,G) At 24 hpf, zic2a and zic5 are coexpressed in the optic stalk, telencephalon and dorsal diencephalon, and weakly in the tectum. All views are lateral, anterior to the left, except in B,C where anterior (a) is at the top. Arrowheads mark anterior and posterior borders of presumptive midbrain. d, dorsal; m, midbrain; h, hindbrain; os, optic stalk; tel, telencephalon.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlx3b WT standard conditions Shield presumptive ectoderm ISH
90%-epiboly non neural ectoderm ISH
zic2a WT standard conditions Shield presumptive neural plate ISH
90%-epiboly neural plate ISH
20-25 somites diencephalon ISH
20-25 somites neural tube ISH
20-25 somites optic stalk ISH
Prim-5 diencephalon ISH
Prim-5 optic stalk ISH
Prim-5 optic tectum ISH
Prim-5 telencephalon ISH
zic5 WT standard conditions 20-25 somites diencephalon ISH
20-25 somites neural tube ISH
20-25 somites optic stalk ISH
Prim-5 diencephalon ISH
Prim-5 optic stalk ISH
Prim-5 optic tectum ISH
Prim-5 telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development