FIGURE

Fig. 4

ID
ZDB-FIG-070307-24
Publication
Nyholm et al., 2007 - The zebrafish zic2a-zic5 gene pair acts downstream of canonical Wnt signaling to control cell proliferation in the developing tectum
Other Figures
All Figure Page
Back to All Figure Page
Fig. 4

Tcf/Lefs are required for zic gene expression. (A) The method used to disrupt Tcf/Lef signaling at controlled times. (B) Temporal correlation between GfpΔTcf induction (by fluorescence) and zic2a RNA (by ISH). (C-L) Representative embryos after ISH. Stage of heat-shock and recovery time are indicated at upper right. Zic2a expression is normal in ΔTcf-negative heat-shocked controls (C,E), and reduced or absent in ΔTcf-positive siblings (D,F). Zic5 expression was normal in ΔTcf-negative (G) embryos, and absent in ΔTcf-positive embryos (H). Zic2aD5:gfp expression was normal in ΔTcf-negative embryos (I) and absent in ΔTcf-positive embryos (J). Wnt3a expression was normal in heat-shocked controls (K) and in ΔTcf-positive siblings (L). All views are lateral, anterior to the left. Arrowheads mark anterior and posterior tectal borders. (M,N) Gfp ChIP analysis of heat-shocked Tg(hs:gfpΔtcf) and Tg(hs:gfp) embryos. Genotype of embryos and PCR templates are shown above, as follows: GFP Ab, chromatin after anti-Gfp IP; input, total chromatin before IP; no Ab, no-antibody ChIP; mock, no chromatin. Regions amplified are labeled next to the corresponding bands. (M) Promoters of known Wnt targets, ngn1 and nacre. An upstream region of ngn1, lacking functional Tcf/Lef-binding sites (lane 5). (N) IG and D5 regions of zic2a-zic5 and zic5 intron.

Expression Data
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: 14-19 somites

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development