ZFIN ID: ZDB-FIG-070605-4
Nyholm et al., 2007 - The zebrafish zic2a-zic5 gene pair acts downstream of canonical Wnt signaling to control cell proliferation in the developing tectum. Development (Cambridge, England)   134(4):735-746 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. S4 Tcf3 reduction affects midbrain size. (A-C) Uninjected control embryos showing normal zic2aD5:gfp (A), zic2a (B) and wnt3a (C) expression at 18-somites. (D-O) Embryos injected with tcf3 MOs and assayed for zic2aD5:gfp (G,J,M), zic2a (H,K,N), or wnt3a (I,L,O) expression at 18-somites. Knockdown of Tcf3 resulted in expanded expression of all markers in the midbrain at low MO concentrations. This is most likely due to the previously documented overall midbrain expansion (D-I). At higher MO concentrations, zic2aD5:gfp and zic2a were reduced in midbrain (J,M,K,N), whereas wnt3a expression remained strong (L,O). Embryos are shown in lateral view, anterior to the left. Arrowheads mark anterior and posterior limits of the midbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
wnt3a WT standard conditions 14-19 somites midbrain ISH
WT + MO1-tcf7l1b + MO2-tcf7l1a standard conditions 14-19 somites midbrain ISH
zic2a WT standard conditions 14-19 somites diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites midbrain ISH
14-19 somites neural tube ISH
14-19 somites optic stalk ISH
14-19 somites telencephalon ISH
WT + MO1-tcf7l1b + MO2-tcf7l1a standard conditions 14-19 somites hindbrain ISH
14-19 somites midbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tcf7l1b + MO2-tcf7l1a standard conditions 14-19 somites midbrain increased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development