ZFIN ID: ZDB-FIG-070307-26
Nyholm et al., 2007 - The zebrafish zic2a-zic5 gene pair acts downstream of canonical Wnt signaling to control cell proliferation in the developing tectum. Development (Cambridge, England)   134(4):735-746 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 14-19 somites

Fig. 6 Multiple Tcf/Lefs are required for zic gene expression. Embryos injected with the MOs shown at the lower left of each panel and stained for zic2aD5:gfp (A,C,E,G,I) or zic2a (B,D,F,H,J) at 18 hpf. (C-D) Strongly affected tcf3 morphants had anterior trunctions and reduced zic2aD5:gfp (asterisks) and zic2a domains. (E-J) lef1 morphants showed a strong reduction in zic2aD5:gfp (E) and a mild reduction in zic2a (F). tcf7 morphants showed a mild reduction in zic2aD5:gfp (G), but normal zic2a (H). lef1+tcf7 morphants showed a strong reduction in zic2aD5:gfp (I) and mild reduction in zic2a (J). All views are lateral, anterior to the left. Arrowheads indicate the midbrain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
zic2a WT control 14-19 somites diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites midbrain ISH
14-19 somites neural tube ISH
14-19 somites optic stalk ISH
14-19 somites telencephalon ISH
WT + MO1-tcf7 standard conditions 14-19 somites diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites midbrain ISH
14-19 somites neural tube ISH
14-19 somites optic stalk ISH
14-19 somites telencephalon ISH
WT + MO1-tcf7 + MO2-lef1 standard conditions 14-19 somites diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites midbrain ISH
14-19 somites neural tube ISH
14-19 somites optic stalk ISH
14-19 somites telencephalon ISH
WT + MO1-tcf7l1b + MO2-tcf7l1a standard conditions 14-19 somites Not Detected diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites midbrain ISH
14-19 somites Not Detected telencephalon ISH
WT + MO2-lef1 standard conditions 14-19 somites diencephalon ISH
14-19 somites hindbrain ISH
14-19 somites midbrain ISH
14-19 somites neural tube ISH
14-19 somites optic stalk ISH
14-19 somites telencephalon ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-tcf7 standard conditions 14-19 somites brain wholly posterioralized, abnormal
14-19 somites midbrain morphology, abnormal
WT + MO1-tcf7l1b + MO2-tcf7l1a standard conditions 14-19 somites forebrain wholly posterioralized, abnormal
WT + MO1-tcf7l1b + MO2-tcf7l1a standard conditions 14-19 somites midbrain decreased size, abnormal
WT + MO2-lef1 standard conditions 14-19 somites brain wholly posterioralized, abnormal
14-19 somites midbrain morphology, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development