ZFIN ID: ZDB-FIG-070307-23
Nyholm et al., 2007
- The zebrafish zic2a-zic5 gene pair acts downstream of canonical Wnt signaling to control cell proliferation in the developing tectum.
Development (Cambridge, England)
134(4):735-746 Full text @ Development
Transient transgenic analysis of zic2a-zic5 genomic regions. (A) Conserved DNA regions (thick lines) at the zic2a-zic5 locus. P2 and P5, each approximately 300 nt, are 70% identical with Fugu. IG (840 nt) is 49% identical to Fugu, and D5 (535 nt) is 60% identical to Fugu. Eight consensus Tcf/Lef-binding sites were found in IG and six in D5 (asterisks). (B) Transgenic constructs tested in transient assays. (C,D) Dorsal midbrain and hindbrain expression of Gfp in representative zic5D5:gfp and zic2aD5:gfp transient transgenics. Insets are dorsal views of the embryos shown. Asterisks mark mid-hindbrain organizer. (E-G) gfp RNA in representative transient transgenics. In embryos injected with 25 pg of zic2aD5:gfp (E) or zic2aD5ΔIG:gfp (F), expression was concentrated in dorsal midbrain and hindbrain. Embryos injected with 25, 50 or 70pg of zic2a:gfp (G) did not show enhanced expression. All views are lateral, except for insets.
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article.
Please note that this material may be protected by copyright.
Full text @ Development