Figure 7—figure supplement 1.

Searching for PDZ-domain containing RhoGEFs potentially involved in the EHT.

(A) Cartoons representing the domains composing the nine PDZ domain-containing RhoGEFs that were investigated in this study (all these RhoGEFs are encoded by different genes in the zebrafish; protein respective length is not drawn at scale). (B) Results obtained from three independent qRT-PCR experiments and performed on material extracted from trunk regions of 35 and 48 hpf embryos obtained from incrossed Tg(kdrl:Gal4;UAS:RFP;4xNR:dt-runx1-eGFP) adult fishes. Statistical tests: two-sided unpaired two samples Wilcoxon test, with p-values of significant differences. GEF: guanine nucleotide-exchange factor; PDZ: postsynaptic density protein of 95 kDa, Discs large and Zona occludens-1; PH: Plekstrin homology; RGS: regulator of G-protein signaling; DH: Dbl (diffuse B-cell lymphoma) homology; RBD: Ras-binding domain; DEP: Dishevelled Egl-10 and Plekstrin; InsPx4-PTPase: PtdIns(1,3,4,5)P4 phosphatidylinositol phosphatase; TIAM: T-cell-lymphoma invasion and metastasis; LARG: leukemia-associated Rho guanine-nucleotide exchange factor; PRex: PtdIns(3,4,5)P3-dependant Rac exchanger-1 and 2.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
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