FIGURE

FIGURE 6

ID

ZDB-FIG-221105-15

Publication

Chen et al., 2022 - TFG mutation induces haploinsufficiency and drives axonal Charcot-Marie-Tooth disease by causing neurite degeneration

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 6

Wnt signaling is activated in response to TFG deficiency. (A) qRT‐PCR‐based validation of the key molecule in Wnt signaling in tfg morphants and control morphants at 3 dpf. (mean ± SD; two‐tailed unpaired t‐test, ***p < 0.001; n = 3). (B) qRT‐PCR‐based validation of the key molecule in Wnt signaling in primary cultured neurons on day 8 and 12 in culture after Tfg knockdown. (mean ± SD; two‐tailed unpaired t‐test; ns, nonsignificant; **p < 0.01; ***p < 0.001; n = 3). (C) Immunofluorescence staining of primary cultured neurons on day 8 after Tfg knockdown and pretreated with LGK974 for 24 h on day 4. Neurites were labeled with MAP2 (green) and the nucleus with DAPI (blue). Scale bars: 20 μm. (D) Quantification of the longest neurite length on day 8 in culture (mean ± SD; two‐tailed unpaired t‐test; ns, nonsignificant; ****p < .0001; n = 60).

Expression Data

Genes:	axin1 axin2 ctnnb1 fzd7a fzd7b lef1 lrp5 lrp6 myca mycn wnt8a wnt9a
Fish:	WT WT + MO1-tfg WT + MO2-tfg
Knockdown Reagents:	MO1-tfg MO2-tfg
Anatomical Term:	whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-tfg WT + MO2-tfg
Knockdown Reagents:	MO1-tfg MO2-tfg
Observed In:	whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ CNS Neurosci Ther