FIGURE

Figure 5

ID

ZDB-FIG-210121-22

Publication

Lu et al., 2021 - Lipin 1 deficiency causes adult-onset myasthenia with motor neuron dysfunction in humans and neuromuscular junction defects in zebrafish

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Figure 5

Lpin 1 deficiency impaired the maturation of schwann cells and myelin synthesis. (A-B') GFP signals of 5 dpf Tg(mbp:eGFP) larvae was visualized by fluorescent microscope, yellow arrows indicated GFP signal in the spinal cord, and red arrows indicated GFP signal motor neurons, lateral views, scale bar: 100 μm. (C) Mbp fluorescence signals of control embryos and lpin1 morphants were analyzed by Image J software. Ten independent samples were evaluated. (D) qRT-PCR measurements of immature SC markers, mature SC markers, and myelin gene markers in lpin1 morphants and STD-MO injected embryos at 3dpf. (E-G) qRT-PCR measurements of pou3f1, egr2b, and mpz dynamic expression at 1 dpf, 3 dpf, and 5 dpf respectively. Values in (D-G) represented means ± SE of data from three independent experiments, **p< 0.01, and ***p < 0.001 (Student's t-test).

Expression Data

Genes:	apoea ccnd1 egr1 egr2a egr2b mpz pmp22a pou3f1
Fish:	ck1Tg ck1Tg + MO2-lpin1a
Knockdown Reagent:	MO2-lpin1a
Anatomical Term:	whole organism
Stage Range:	Protruding-mouth to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ck1Tg + MO2-lpin1a
Knockdown Reagent:	MO2-lpin1a
Observed In:	myelination oligodendrocyte differentiation Schwann cell differentiation whole organism
Stage Range:	Protruding-mouth to Day 5

Phenotype Detail

Acknowledgments

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