Figure 6

Locomotor deficits in lpin1 morphants. (A-C)lpin1 morphant embryos exhibited reduced touch-evoked response at 4 dpf (B) while control embryos swam away rapidly after mechanosensory stimulation (A). The reduced touch-evoked response of lpin1 morphant could be rescued by co-injected hLPIN1^wt mRNA (C). Scale bar: 200 µm. (D) Cumulative plots of the position and velocity of control larva, lpin1 morphants, and lpin1 morphants co-injected with hLPIN1^wt mRNA; during 5 min of behavioral recording. 8 representative individuals were recorded for each treatment group. (E) Representative heat map plots. Red dots indicate the long rest time without any movement. (F) Cumulative duration records showed that the lpin1 morphants were less active. (G) Velocity was dramatically decreased in lpin1 morphants, but can be rescued by the hLPIN1^wt mRNA. (H) Total movement distance during a 5 min startle response analysis as follows: 20 s spontaneous movement tracking followed by cycle stimulation with light on 5 s, interval 1 s, trigger tap 1 s, and interval 48 s; repeated 5 cycles. Data shown are means of distance moved in 5 s ± SE (n = 24 for each group at 5 dpf).