FIGURE

Figure 4

ID

ZDB-FIG-210121-21

Publication

Lu et al., 2021 - Lipin 1 deficiency causes adult-onset myasthenia with motor neuron dysfunction in humans and neuromuscular junction defects in zebrafish

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Figure 4

Knockdown of lpin1 in zebrafish embryos affected neuron development and AChR clustering. Znp1 antibody staining (A-B) and α-BTX labeling (A'-B') of control embryos (A-A'') and lpin1 morphants (B-B'') at 26 hpf. Zn8 antibody staining (C-D) and α-BTX labeling (C'-D') of control embryos (C-C'') and lpin1 morphants (D-D'') at 60 hpf. Scale bar: 100 µm. Measurements of the length of motor neurons (E, n = 6 motor neurons/larvae), the distance between two adjacent motor neurons (F, n = 6 segments/larvae), the angle of AChR clusters (G, n = 6 AChR clusters/larvae), and the density of post-synapses (H, n = 6 areas of myotome/larvae), were carried out in 9 larva under each treatment. (I) Schematic representations of the parameters in E, F, G and H. *** indicates p < 0.001 by Student's t-test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO2-lpin1a
Knockdown Reagent:	MO2-lpin1a
Observed In:	motor neuron axon motor neuron axon guidance neuromuscular junction development primary motor neuron neuron projection secondary motor neuron secondary motor neuron axon skeletal muscle acetylcholine-gated channel clustering (all 7)
Stage Range:	Prim-5 to Pec-fin

Phenotype Detail

Acknowledgments

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