ZFIN ID: ZDB-FIG-200829-85
Zhang et al., 2020 - THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis. PLoS Genetics   16:e1008953 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Long-pec to Day 4

Fig 6 Transcriptome sequencing analysis of 48 hpf <italic>thoc1</italic> mutants.

(a) Clustering analysis indicates the replicates within group have a good repeatability, while the control and mutated group are different. (b) Volcano diagram of different expression genes. Red dots indicate up-regulated genes; blue dots indicate down-regulated genes. Abscissa indicates gene fold change in different samples; ordinate represents statistical significance of gene expression change. (c) KEGG analysis plot of the differential gene, with the vertical axis representing the pathway and the horizontal axis representing the Rich factor. The size of the dot indicates the number of differentially expressed genes in the pathway, and the color of the dot corresponds to a different Qvalue range. (d) Relative mRNA levels of p53 in control and thoc1-KO embryos at 48 hpf and 4 dpf (three times experiments, n = 10 for each time). t-test; ***, p<0.001; ****, p<0.0001. (e) Relative mRNA levels of bax, casp3 and casp9 in control and thoc1-KO embryos at 4 dpf (three times experiments, n = 10 for each time), t-test; **, p<0.01; ***, p<0.001; ****, p<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
baxa s356tTg control Day 4 whole organism RTPCR
s356tTg + CRISPR1-thoc1 standard conditions Day 4 whole organism RTPCR
casp3a s356tTg control Day 4 whole organism RTPCR
s356tTg + CRISPR1-thoc1 standard conditions Day 4 whole organism RTPCR
casp9 s356tTg control Day 4 whole organism RTPCR
s356tTg + CRISPR1-thoc1 standard conditions Day 4 whole organism RTPCR
tp53 s356tTg control Long-pec whole organism RTPCR
Day 4 whole organism RTPCR
s356tTg + CRISPR1-thoc1 standard conditions Long-pec whole organism RTPCR
Day 4 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + CRISPR1-thoc1 standard conditions Long-pec whole organism tp53 expression increased amount, abnormal
Day 4 whole organism baxa expression increased amount, abnormal
Day 4 whole organism casp3a expression increased amount, abnormal
Day 4 whole organism casp9 expression increased amount, abnormal
Day 4 whole organism tp53 expression increased amount, abnormal
Acknowledgments:
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