ZFIN ID: ZDB-FIG-200829-81
Zhang et al., 2020 - THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis. PLoS Genetics   16:e1008953 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig 3 <italic>Thoc1</italic> deficiency caused hair cell developmental defects in zebrafish.

(a) Fluorescence microscopic imaging analysis of thoc1 mutant Tg(pou4f3:gap43-GFP) line at 3 dpf. Arrowheads indicate hair cell clusters. (b) Statistical analysis of the hair cell clusters in control and thoc1 mutants (control, n = 10; thoc1 mutants, n = 37). t-test, ****, p<0.0001. (c) Fluorescence microscopic imaging analysis of thoc1 mutant Tg(pou4f3:gap43-GFP) line at 4 dpf. Arrowheads indicate hair cell clusters. (d) Statistical analysis of the hair cell clusters in control and thoc1 mutants (control, n = 10; thoc1 mutants, n = 38). t-test, ****, p<0.0001. (e) Confocal microscopic imaging analysis of the neuromasts in control and thoc1 mutants at 3 dpf. Green: Tg(pou4f3:gap43-GFP). Red: Sox2 antibody staining marking supporting cells. Blue: DAPI staining of the cell nuclei. (f, g) Statistical analysis of the hair cell number per neuromasts and total number in trunk of control and thoc1 mutants (control, n = 9; thoc1 mutants, n = 17, control, n = 15; thoc1 mutants, n = 15) at 3 dpf. t-test, ****, p<0.0001. (h) Confocal microscopic imaging analysis of the neuromasts in control and thoc1 mutants at 4 dpf. Green: Tg(pou4f3:gap43-GFP). Red: Sox2 antibody staining marking supporting cells. Blue: DAPI staining of the cell nuclei. (i, j) Statistical analysis of the hair cell number per neuromasts and total number in trunk of control and thoc1 mutants (control, n = 9, thoc1 mutants, n = 17; control, n = 15; thoc1 mutants, n = 15) at 4 dpf. t-test, ****, p<0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + CRISPR1-thoc1 standard conditions Protruding-mouth neuromast decreased amount, abnormal
Protruding-mouth neuromast hair cell decreased amount, abnormal
Protruding-mouth neuromast has fewer parts of type neuromast hair cell, abnormal
Day 4 neuromast decreased amount, abnormal
Day 4 neuromast hair cell decreased amount, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.