ZFIN ID: ZDB-FIG-200829-86
Zhang et al., 2020 - THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis. PLoS Genetics   16:e1008953 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage: Day 4

Fig 7 Inhibition of P53 signaling alleviated the <italic>thoc1</italic>-deficiency induced apoptosis in neuromasts.

(a, b) Statistical analysis of the hair cell clusters in control (n = 8), ctrl + P53 inhibitor treatment (n = 9), thoc1 KO (n = 8), and thoc1 KO + P53 inhibitor treatment embryos (n = 7). One-way ANOVA, ****, p<0.0001; ***, p<0.001; **, p<0.01. (c) TUNEL analysis of the neuromasts in control (n = 7), thoc1 KO (n = 7), and thoc1 KO + P53 inhibitor treatment embryos (n = 7). Green: TUNEL staining. Blue: DAPI staining of the cell nuclei. (d) Statistical analysis of the number of apoptotic cells per neuromast in control, thoc1 KO, and thoc1 KO + P53 inhibitor treatment embryos. One-way ANOVA, ****, p<0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + CRISPR1-thoc1 control Day 4 neuromast hair cell apoptotic process increased occurrence, abnormal
Day 4 neuromast hair cell decreased amount, abnormal
s356tTg + CRISPR1-thoc1 chemical treatment: pharmaceutical Day 4 neuromast hair cell amount, ameliorated
Day 4 neuromast hair cell apoptotic process occurrence, ameliorated
Acknowledgments:
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