ZFIN ID: ZDB-FIG-200829-84
Zhang et al., 2020 - THOC1 deficiency leads to late-onset nonsyndromic hearing loss through p53-mediated hair cell apoptosis. PLoS Genetics   16:e1008953 Full text @ PLoS Genet.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig 5 Inactivation of <italic>thoc1</italic> induced apoptosis in neuromasts.

(a) Confocal microscopic imaging analysis of the hair cells in control and thoc1-KO Tg(pou4f3:gap43-GFP) at 3 and 4 dpf. Arrowheads indicate the abnormal hair cells. (b) TUNEL analysis of the neuromasts in control and thoc1-KO embryos at 4 dpf. Green: TUNEL staining. Blue: DAPI staining of the cell nuclei.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
s356tTg + CRISPR1-thoc1 standard conditions Protruding-mouth neuromast hair cell decreased size, abnormal
Protruding-mouth neuromast hair cell deformed, abnormal
Protruding-mouth neuromast hair cell morphology, abnormal
Day 4 neuromast hair cell decreased size, abnormal
Day 4 neuromast hair cell deformed, abnormal
Day 4 neuromast hair cell morphology, abnormal
Day 4 neuromast hair cell shape, abnormal
Day 4 neuromast support cell apoptotic, abnormal
Acknowledgments:
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.