ZFIN ID: ZDB-FIG-200805-27
Espín-Palazón et al., 2014 - Proinflammatory signaling regulates hematopoietic stem cell emergence. Cell   159:1070-85 Full text @ Cell
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-25 to Day 4

Fig. 1 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP s896Tg; zf169Tg standard conditions Long-pec endothelial cell IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
s896Tg; zf169Tg + MO2-tnfrsf1b standard conditions Long-pec endothelial cell IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
s896Tg; zf169Tg + MO4-tnfa standard conditions Long-pec endothelial cell IFL
Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
foxn1 WT standard conditions Day 4 thymic epithelium ISH
WT + MO2-tnfrsf1b standard conditions Day 4 thymic epithelium ISH
WT + MO4-tnfa standard conditions Day 4 thymic epithelium ISH
mCherry s896Tg; zf169Tg standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
s896Tg; zf169Tg + MO2-tnfrsf1b standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
s896Tg; zf169Tg + MO4-tnfa standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell IFL
myb WT standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO2-tnfrsf1a standard conditions Long-pec ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO2-tnfrsf1a + MO2-tnfrsf1b standard conditions Long-pec ventral wall of dorsal aorta ISH
Long-pec ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO2-tnfrsf1b standard conditions Long-pec ventral wall of dorsal aorta ISH
Long-pec ventral wall of dorsal aorta hematopoietic stem cell ISH
WT + MO4-tnfa standard conditions Long-pec ventral wall of dorsal aorta ISH
Long-pec ventral wall of dorsal aorta hematopoietic stem cell ISH
rag1 WT standard conditions Day 4 T cell ISH
WT + MO2-tnfrsf1b standard conditions Day 4 Not Detected T cell ISH
WT + MO4-tnfa standard conditions Day 4 Not Detected T cell ISH
tnfrsf1b s896Tg; zf169Tg standard conditions Long-pec hematopoietic stem cell RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-tnfrsf1a + MO2-tnfrsf1b standard conditions Long-pec ventral wall of dorsal aorta myb expression decreased distribution, abnormal
Long-pec ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
WT + MO2-tnfrsf1b standard conditions Long-pec ventral wall of dorsal aorta myb expression decreased distribution, abnormal
Long-pec ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
Day 4 T cell rag1 expression absent, abnormal
WT + MO4-tnfa standard conditions Long-pec ventral wall of dorsal aorta myb expression decreased distribution, abnormal
Long-pec ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
Day 4 T cell rag1 expression absent, abnormal
la2Tg + MO2-tnfrsf1b standard conditions Protruding-mouth hematopoietic multipotent progenitor cell decreased amount, abnormal
la2Tg + MO4-tnfa standard conditions Protruding-mouth hematopoietic multipotent progenitor cell decreased amount, abnormal
s896Tg; zf169Tg + MO2-tnfrsf1b standard conditions Prim-25 - Long-pec hematopoietic stem cell homeostasis decreased occurrence, abnormal
Long-pec ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
s896Tg; zf169Tg + MO4-tnfa standard conditions Prim-25 - Long-pec hematopoietic stem cell homeostasis decreased occurrence, abnormal
Long-pec ventral wall of dorsal aorta hematopoietic stem cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Cell for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Cell, 159, Espín-Palazón, R., Stachura, D.L., Campbell, C.A., García-Moreno, D., Del Cid, N., Kim, A.D., Candel, S., Meseguer, J., Mulero, V., Traver, D., Proinflammatory signaling regulates hematopoietic stem cell emergence, 1070-85, Copyright (2014) with permission from Elsevier. Full text @ Cell