Caspy2 is critical for lethal sepsis in zebrafish larvae.
(a-c) Controlmorpholino oligonucleotide (MO) or caspy2-MO zebrafish larvae at 5 dpf were immersed in 100 μg/ml of LPS. (a) Survival was then monitored for 72 hpi. Results are representative of at least three independent experiments. ** p < 0.01 (log-rank). (b) Distinct pathologies associated with lethal dose of LPS. Pericardial edema (red arrow); Fin erosion and ulceration (black arrow); Protrusions on the trunk or tail regions (white arrow). Results are representative of at least three independent experiments. (c) RT-PCR analysis of the specified cytokine transcripts at the indicated time points in control- and caspy2-MO larvae. Each bar represents mean results SEM from 3-4 pools of 15 larvae. RT-PCR for each pool was carried out using technical duplicates. *p < 0.05, **p < 0.01 (t-test).