ZFIN ID: ZDB-FIG-180913-19
Yang et al., 2018 - Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Nature communications   9:3052 Full text @ Nat. Commun.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Term:
Stage Range: Day 5 to Days 7-13
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. S10

Caspy2 is critical for lethal sepsis in zebrafish larvae.

(a-c) Controlmorpholino oligonucleotide (MO) or caspy2-MO zebrafish larvae at 5 dpf were immersed in 100 μg/ml of LPS. (a) Survival was then monitored for 72 hpi. Results are representative of at least three independent experiments. ** p < 0.01 (log-rank). (b) Distinct pathologies associated with lethal dose of LPS. Pericardial edema (red arrow); Fin erosion and ulceration (black arrow); Protrusions on the trunk or tail regions (white arrow). Results are representative of at least three independent experiments. (c) RT-PCR analysis of the specified cytokine transcripts at the indicated time points in control- and caspy2-MO larvae. Each bar represents mean results SEM from 3-4 pools of 15 larvae. RT-PCR for each pool was carried out using technical duplicates. *p < 0.05, **p < 0.01 (t-test).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
cxcl8a AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
AB + MO1-caspb chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
ifng1 AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
AB + MO1-caspb chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
il1b AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
AB + MO1-caspb chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
il6 AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
AB + MO1-caspb chemical treatment by environment: lipopolysaccharide Days 7-13 whole organism RTPCR
il10 AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
AB + MO1-caspb chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
tnfa AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism RTPCR
Days 7-13 whole organism RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB chemical treatment by environment: lipopolysaccharide Day 5 whole organism cxcl8a expression increased amount, abnormal
Day 5 whole organism ifng1 expression increased amount, abnormal
Day 5 whole organism il10 expression increased amount, abnormal
Day 5 whole organism il1b expression increased amount, abnormal
Day 5 whole organism il6 expression increased amount, abnormal
Day 5 whole organism tnfa expression increased amount, abnormal
Day 5 - Days 7-13 caudal fin morphology, abnormal
Day 5 - Days 7-13 fin eroding, abnormal
Day 5 - Days 7-13 pericardial region edematous, abnormal
Day 5 - Days 7-13 trunk morphology, abnormal
Day 5 - Days 7-13 whole organism decreased life span, abnormal
Days 7-13 whole organism il1b expression increased amount, abnormal
Days 7-13 whole organism il6 expression amount, ameliorated
Days 7-13 whole organism tnfa expression amount, ameliorated
AB + MO1-caspb chemical treatment by environment: lipopolysaccharide Day 5 whole organism cxcl8a expression amount, ameliorated
Day 5 whole organism ifng1 expression amount, ameliorated
Day 5 whole organism il10 expression amount, ameliorated
Day 5 whole organism il1b expression amount, ameliorated
Day 5 - Days 7-13 caudal fin morphology, ameliorated
Day 5 - Days 7-13 fin eroding, ameliorated
Day 5 - Days 7-13 pericardial region edematous, ameliorated
Day 5 - Days 7-13 trunk morphology, ameliorated
Day 5 - Days 7-13 whole organism decreased life span, ameliorated
Days 7-13 whole organism ifng1 expression increased amount, abnormal
Days 7-13 whole organism il10 expression increased amount, abnormal
Days 7-13 whole organism il1b expression amount, ameliorated
Days 7-13 whole organism il6 expression increased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.