FIGURE

Fig. 2

ID

ZDB-FIG-180913-12

Publication

Yang et al., 2018 - Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish

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Fig. 2

Caspy2 plays a critical role in pyroptosis in zebrafish fibroblasts. a–c Wild-type and caspy2-knockout (KO) ZF4 cells were infected with EIB202 or 0909I E. piscicida for 2 h at an multiplicity of infection (MOI) of 50, or left uninfected. a Images were taken as in Fig. 1d. Propidium iodide (PI) was added to detect the loss of plasma membrane integrity. Arrows signify cells with pyroptotic-like features. Scale bar, 50 µm. b Supernatants from the indicated ZF4 cells were analyzed for cell death measured by lactate dehydrogenase (LDH) release. c Mixtures of cell lysates and supernatants were subjected to immunoblotting. (a–c) Results are representative of at least three independent experiments, and error bars denote the SD of triplicate wells. ^*p < 0.05 (t test)

Expression Data

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Acknowledgments

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