ZFIN ID: ZDB-FIG-180913-16
Yang et al., 2018 - Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Nature communications   9:3052 Full text @ Nat. Commun.
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 6

Caspy2 restricts bacterial colonization of the zebrafish gut in vivo. ae Control-morpholino oligonucleotide (MO) or caspy2-MO zebrafish larvae at 5 days postfertilization (dpf) were infected by immersion with 105 colony forming units (CFU)/ml of 0909I E. piscicida. a Zebrafish larvae (5 dpf) were infected by immersion with wild-type (EIB202) or 0909I E. piscicida. Immunoblotting analysis of caspy2 levels at the indicated post-infection time points. b Survival of zebrafish larvae was monitored for 4 days. (n = 60 for control-MO, n = 60 for caspy2-MO). Results are representative of at least three independent experiments, **p < 0.01 (log-rank). c Zebrafish larvae were collected at the indicated postinfection time points, and homogenates were made for CFU counts. Each symbol represents the average counts of five larvae (n = 100 for control-MO, n = 100 for caspy2-MO). Results are representative of at least three independent experiments. *p < 0.05 (ANOVA). d Images of infected zebrafish larvae. Green fluorescent protein (GFP)-0909I E. piscicida. e Representative images of hematoxylin and eosin (H&E) staining of gut sections from 0909I-infected control-MO or caspy2-MO zebrafish larvae. Square frame, intestinal wall morphology; Scale bar, 10 µm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-caspb bacterial treatment by exposure to environment: Edwardsiella piscicida Day 5 - Days 7-13 defense response to bacterium decreased efficacy, abnormal
Day 5 - Days 7-13 whole organism decreased life span, abnormal
Days 7-13 gut inflammatory response increased process quality, abnormal
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.