ZFIN ID: ZDB-FIG-180913-16
Yang et al., 2018 - Sensing of cytosolic LPS through caspy2 pyrin domain mediates noncanonical inflammasome activation in zebrafish. Nature communications   9:3052 Full text @ Nat. Commun.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Condition:
Knockdown Reagent:
Observed In:
Stage Range: Day 5 to Days 7-13

Fig. 6

Caspy2 restricts bacterial colonization of the zebrafish gut in vivo. ae Control-morpholino oligonucleotide (MO) or caspy2-MO zebrafish larvae at 5 days postfertilization (dpf) were infected by immersion with 105 colony forming units (CFU)/ml of 0909I E. piscicida. a Zebrafish larvae (5 dpf) were infected by immersion with wild-type (EIB202) or 0909I E. piscicida. Immunoblotting analysis of caspy2 levels at the indicated post-infection time points. b Survival of zebrafish larvae was monitored for 4 days. (n = 60 for control-MO, n = 60 for caspy2-MO). Results are representative of at least three independent experiments, **p < 0.01 (log-rank). c Zebrafish larvae were collected at the indicated postinfection time points, and homogenates were made for CFU counts. Each symbol represents the average counts of five larvae (n = 100 for control-MO, n = 100 for caspy2-MO). Results are representative of at least three independent experiments. *p < 0.05 (ANOVA). d Images of infected zebrafish larvae. Green fluorescent protein (GFP)-0909I E. piscicida. e Representative images of hematoxylin and eosin (H&E) staining of gut sections from 0909I-infected control-MO or caspy2-MO zebrafish larvae. Square frame, intestinal wall morphology; Scale bar, 10 µm

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-caspb bacterial treatment by exposure to environment: Edwardsiella piscicida Day 5 - Days 7-13 defense response to bacterium decreased efficacy, abnormal
Day 5 - Days 7-13 whole organism decreased life span, abnormal
Days 7-13 gut inflammatory response increased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Nature communications for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Nat. Commun.