Fig. 6

Caspy2 restricts bacterial colonization of the zebrafish gut in vivo. a–e Control-morpholino oligonucleotide (MO) or caspy2-MO zebrafish larvae at 5 days postfertilization (dpf) were infected by immersion with 10⁵ colony forming units (CFU)/ml of 0909I E. piscicida. a Zebrafish larvae (5 dpf) were infected by immersion with wild-type (EIB202) or 0909I E. piscicida. Immunoblotting analysis of caspy2 levels at the indicated post-infection time points. b Survival of zebrafish larvae was monitored for 4 days. (n = 60 for control-MO, n = 60 for caspy2-MO). Results are representative of at least three independent experiments, ^**p < 0.01 (log-rank). c Zebrafish larvae were collected at the indicated postinfection time points, and homogenates were made for CFU counts. Each symbol represents the average counts of five larvae (n = 100 for control-MO, n = 100 for caspy2-MO). Results are representative of at least three independent experiments. ^*p < 0.05 (ANOVA). d Images of infected zebrafish larvae. Green fluorescent protein (GFP)-0909I E. piscicida. e Representative images of hematoxylin and eosin (H&E) staining of gut sections from 0909I-infected control-MO or caspy2-MO zebrafish larvae. Square frame, intestinal wall morphology; Scale bar, 10 µm