ZFIN ID: ZDB-FIG-180719-2
Tokunaga et al., 2017 - Comprehensive validation of T- and B-cell deficiency in rag1-null zebrafish: Implication for the robust innate defense mechanisms of teleosts. Scientific Reports   7:7536 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage: Adult

Unillustrated author statements

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
ifng1 rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
ifng1r rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
ifnphi1 rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
il1b rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
il10 rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
il12a rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
il13 rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
il17a/f2 rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
il17a/f3 rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
tnfa rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
tnfb rag1t26683/+ chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney RTPCR
Adult liver RTPCR
Adult pancreas RTPCR
Adult spleen RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
rag1t26683/+ chemical treatment by injection: poly(I:C) Adult liver il10 expression decreased amount, abnormal
Adult liver il13 expression decreased amount, abnormal
Adult pancreas il10 expression decreased amount, abnormal
Adult pancreas il13 expression decreased amount, abnormal
Adult spleen il10 expression increased amount, abnormal
Adult spleen il13 expression increased amount, abnormal
Adult spleen tnfa expression increased amount, abnormal
rag1t26683/t26683 standard conditions Adult inflammatory response to antigenic stimulus decreased efficacy, abnormal
Adult kidney granulocyte increased amount, abnormal
Adult mature B cell absent, abnormal
Adult whole organism lacks parts or has fewer parts of type blood IgM immunoglobulin complex, abnormal
rag1t26683/t26683 chemical treatment by injection: poly(I:C) Adult kidney granulocyte increased amount, abnormal
Adult liver il10 expression increased amount, abnormal
Adult liver il13 expression increased amount, abnormal
Adult liver il17a/f2 expression increased amount, abnormal
Adult liver il17a/f3 expression increased amount, abnormal
Adult liver macrophage increased amount, abnormal
Adult pancreas il10 expression increased amount, abnormal
Adult pancreas il13 expression increased amount, abnormal
Adult pancreas il17a/f2 expression increased amount, abnormal
Adult pancreas il17a/f3 expression increased amount, abnormal
Adult pancreas macrophage increased amount, abnormal
Adult spleen tnfa expression decreased amount, abnormal
Adult spleen granulocyte increased amount, abnormal
Adult spleen macrophage increased amount, abnormal
rag1t26683/t26683 bacterial treatment by exposure to environment: Vibrio anguillarum Adult defense response to bacterium disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.