ZFIN ID: ZDB-FIG-180711-34
Selland et al., 2018 - Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development. Mechanisms of Development   150:28-41 Full text @ Mech. Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage: 1-4 somites
PHENOTYPE:
Fish:
Observed In:
Stage: 1-4 somites

Fig. 9

Hoxb1b regulates FGF signaling.

Fgf3 is expressed in r4 in wildtype embryos (A) and hoxb1a−/− mutants (B). Fgf3 expression is lost in hoxb1b−/− mutants (16/16 embryos) (C) and hoxb1a−/−;hoxb1b−/− mutants (4/4 embryos) (D). Fgf8 is expressed in r4, medial r2 and the MHB (E). The loss of hoxb1a reduces the space between r2 and r4 and the MHB becomes arrowhead shaped (14/19 embryos) (F). In hoxb1b−/− mutants (19/19 embryos) (G) and hoxb1a−/−hoxb1b−/− mutants (8/8 embryos) (H) there is ectopic expression of fgf8 in r3 and reduced expression in the lateral portions of r4. The expression of FGF responsive genes, pea3 (I–L), dusp6 (M–P), spry1 (Q–T), spry2 (U–X), and spry4 (Y–B′) are unaltered from wildtype (I,M,Q,U,Y) in hoxb1a−/− mutants (J,N,R,V,Z). In both hoxb1b−/− mutants (pea3: 6/7 embryos, dusp6: 10/11 embryos, spry1: 25/25 embryos, spry2: 19/19 embryos, spry4: 23/23 embryos) (K,O,S,W,A') and hoxb1a−/−;hoxb1b−/− mutants (pea3: 6/6 embryos, dusp6: 5/5 embryos, spry1: 9/9 embryos, spry2: 5/5 embryos, spry4: 4/4 embryos) (L,P,T,X,B′) there is an increase in expression levels. Spry1 and spry2 show ectopic expression in r3 (S,T,W,X), while spry4 is ectopically expressed in r4 (A′, B′). All embryos have been genotyped for hoxb1asa1191 and hoxb1bua1006. All images are dorsal views, anterior to the left, 4 somites.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dusp6 AB standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
egr2b AB standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
etv4 AB standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites hindbrain neural plate ISH
1-4 somites midbrain hindbrain boundary neural keel ISH
fgf3 AB standard conditions 1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites presumptive rhombomere 4 ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites presumptive rhombomere 4 ISH
fgf8a AB standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
spry1 AB standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
spry2 AB standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 2 ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
spry4 AB standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxb1asa1191/sa1191 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
1-4 somites presumptive rhombomere 5 ISH
hoxb1bua1006/ua1006 standard conditions 1-4 somites midbrain hindbrain boundary neural keel ISH
1-4 somites presumptive rhombomere 3 ISH
1-4 somites presumptive rhombomere 4 ISH
1-4 somites presumptive rhombomere 5 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
hoxb1asa1191/sa1191 standard conditions 1-4 somites midbrain hindbrain boundary neural keel morphology, abnormal
1-4 somites midbrain hindbrain boundary neural keel fgf8a expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 2 fgf8a expression spatial pattern, abnormal
hoxb1asa1191/sa1191 ; hoxb1bua1006/ua1006 standard conditions 1-4 somites hindbrain neural plate dusp6 expression spatial pattern, abnormal
1-4 somites hindbrain neural plate etv4 expression spatial pattern, abnormal
1-4 somites midbrain hindbrain boundary neural keel morphology, abnormal
1-4 somites midbrain hindbrain boundary neural keel dusp6 expression spatial pattern, abnormal
1-4 somites midbrain hindbrain boundary neural keel etv4 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 3 spry2 expression mislocalised, abnormal
1-4 somites presumptive rhombomere 3 fgf8a expression mislocalised, abnormal
1-4 somites presumptive rhombomere 3 spry1 expression mislocalised, abnormal
1-4 somites presumptive rhombomere 3 spry2 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 3 fgf8a expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 3 spry1 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 4 fgf3 expression decreased amount, abnormal
1-4 somites presumptive rhombomere 4 fgf3 expression decreased distribution, abnormal
1-4 somites presumptive rhombomere 4 decreased size, abnormal
1-4 somites presumptive rhombomere 4 spry4 expression mislocalised, abnormal
1-4 somites presumptive rhombomere 4 morphology, abnormal
1-4 somites presumptive rhombomere 4 spry4 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 4 fgf8a expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 5 egr2b expression decreased amount, abnormal
1-4 somites presumptive rhombomere 5 egr2b expression decreased distribution, abnormal
1-4 somites presumptive rhombomere 5 decreased size, abnormal
1-4 somites presumptive rhombomere 5 morphology, abnormal
hoxb1bua1006/ua1006 standard conditions 1-4 somites hindbrain neural plate dusp6 expression spatial pattern, abnormal
1-4 somites hindbrain neural plate etv4 expression spatial pattern, abnormal
1-4 somites midbrain hindbrain boundary neural keel dusp6 expression spatial pattern, abnormal
1-4 somites midbrain hindbrain boundary neural keel etv4 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 3 spry2 expression mislocalised, abnormal
1-4 somites presumptive rhombomere 3 spry1 expression mislocalised, abnormal
1-4 somites presumptive rhombomere 3 fgf8a expression mislocalised, abnormal
1-4 somites presumptive rhombomere 3 spry2 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 3 spry1 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 3 fgf8a expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 4 fgf3 expression decreased amount, abnormal
1-4 somites presumptive rhombomere 4 fgf3 expression decreased distribution, abnormal
1-4 somites presumptive rhombomere 4 decreased size, abnormal
1-4 somites presumptive rhombomere 4 spry4 expression mislocalised, abnormal
1-4 somites presumptive rhombomere 4 morphology, abnormal
1-4 somites presumptive rhombomere 4 spry4 expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 4 fgf8a expression spatial pattern, abnormal
1-4 somites presumptive rhombomere 5 egr2b expression decreased amount, abnormal
1-4 somites presumptive rhombomere 5 egr2b expression decreased distribution, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.