FIGURE

Fig. S5

ID

ZDB-FIG-180711-40

Publication

Selland et al., 2018 - Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development

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Fig. S5

Two-color analysis of rhombomere identity with fgf8, dusp6 and spry2.

The expression of representative markers is examined in combination with krox20. Fgf8, dusp6 and spry2 are shown in purple, while krox20 is shown in red. Krox20 expression labels r3 and r5. All embryos are dorsal views, anterior to the left, 4–5 somites. Fgf8 is expressed in r4, medial r2 and the MHB (A). The loss of hoxb1a reduces the space between r2 and r4 and the MHB becomes arrowhead shaped (B). In hoxb1b^−/− mutants (C) and hoxb1a^−/−hoxb1b^−/− mutants (D) there is ectopic expression of fgf8 in r3 and reduced expression in the lateral portions of r4. The expression of FGF responsive genes, dusp6 (E-H), and spry2 (I-L), are unaltered from wildtype (E,I) in hoxb1a^−/− mutants (F,J). In both hoxb1b^−/− mutants (G,K) and hoxb1a^−/−;hoxb1b^−/− mutants (H,L) there is an increase in expression levels. Spry2 shows ectopic expression in r3 (K,L). All embryos have been genotyped for hoxb1a^sa1191 and hoxb1b^ua1006. All images are dorsal views, anterior to the left, 4 somites.

Expression Data

Genes:	egr2b fgf8a spry2
Fish:	AB hoxb1a^{sa1191/sa1191} hoxb1b^{ua1006/ua1006} hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Anatomical Terms:	midbrain hindbrain boundary neural keel presumptive rhombomere 2 presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hoxb1a^{sa1191/sa1191} hoxb1b^{ua1006/ua1006} hoxb1a^{sa1191/sa1191}; hoxb1b^{ua1006/ua1006}
Observed In:	midbrain hindbrain boundary neural keel presumptive rhombomere 3 presumptive rhombomere 4 presumptive rhombomere 5
Stage:	5-9 somites

Phenotype Detail

Acknowledgments

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Reprinted from Mechanisms of Development, 150, Selland, L.G., Koch, S., Laraque, M., Waskiewicz, A.J., Coordinate regulation of retinoic acid synthesis by pbx genes and fibroblast growth factor signaling by hoxb1b is required for hindbrain patterning and development, 28-41, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.