FIGURE

Fig. 3-S1

ID
ZDB-FIG-180417-23
Publication
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile
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Fig. 3-S1

Expression of nodal, lefty, and Nodal target genes in lefty1-/-;lefty2-/- mutants during gastrulation.

Wild type embryos, lft1-/- embryos, lft2-/- embryos, and (to generate lft1-/-;lft2-/- embryos) progeny from lft1+/-;lft2-/- incrosses, lft1-/-;lft2-/- incrosses, or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses were fixed at the indicated stages. In situ hybridization was performed using probes against the following Nodal target genes: lft1 (A), lft2 (B), sqt (C), cyc (D), tb/no tail (E), and sox17 (F). Dorsal views. After imaging, embryos from lft1+/-;lft2-/- incrosses or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses were genotyped (see Materials and methods for details). Upregulation of Nodal target genes was most dramatic in lft1-/-;lft2-/- mutants, though lft1-/- mutants tended to exhibit slight upregulation as well. Note that expression of tb/no tail at 30% epiboly was normal in lft1-/-;lft2-/- mutants, in contrast to the dramatic upregulation at 30% epiboly observed in double lefty morphants (Agathon et al., 2001).

Expression Data
Genes:
Fish:
Anatomical Terms:
Stage Range: Sphere to 75%-epiboly

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Observed In:
Stage Range: Sphere to 75%-epiboly

Phenotype Detail
Acknowledgments
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