ZFIN ID: ZDB-FIG-180417-23
Rogers et al., 2017 - Nodal patterning without Lefty inhibitory feedback is functional but fragile. eLIFE   6 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Sphere to 75%-epiboly
PHENOTYPE:
Fish:
Observed In:
Stage Range: Sphere to 75%-epiboly

Fig. 3-S1

Expression of nodal, lefty, and Nodal target genes in lefty1-/-;lefty2-/- mutants during gastrulation.

Wild type embryos, lft1-/- embryos, lft2-/- embryos, and (to generate lft1-/-;lft2-/- embryos) progeny from lft1+/-;lft2-/- incrosses, lft1-/-;lft2-/- incrosses, or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses were fixed at the indicated stages. In situ hybridization was performed using probes against the following Nodal target genes: lft1 (A), lft2 (B), sqt (C), cyc (D), tb/no tail (E), and sox17 (F). Dorsal views. After imaging, embryos from lft1+/-;lft2-/- incrosses or lft1-/-;lft2-/- X lft1+/-;lft2-/- crosses were genotyped (see Materials and methods for details). Upregulation of Nodal target genes was most dramatic in lft1-/-;lft2-/- mutants, though lft1-/- mutants tended to exhibit slight upregulation as well. Note that expression of tb/no tail at 30% epiboly was normal in lft1-/-;lft2-/- mutants, in contrast to the dramatic upregulation at 30% epiboly observed in double lefty morphants (Agathon et al., 2001).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lft1 WT standard conditions Sphere blastodisc ISH
Shield margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft1a145/a145 standard conditions Sphere blastodisc anatomical margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc anatomical margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
75%-epiboly margin ISH
lft2 WT standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
Shield margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly axis ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly axis ISH
ndr1 WT standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
ndr2 WT standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
50%-epiboly margin ISH
75%-epiboly axis ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly axis ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly axis ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly axis ISH
sox17 WT standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
Shield margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly endoderm ISH
lft1a145/a145 ; lft2a146/a146 standard conditions 50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly endoderm ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly endoderm ISH
tbxta WT standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 standard conditions Sphere blastodisc ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
Shield margin ISH
75%-epiboly margin ISH
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc ISH
30%-epiboly margin ISH
Shield margin ISH
50%-epiboly margin ISH
75%-epiboly margin ISH
lft2a146/a146 standard conditions Sphere blastodisc ISH
Shield margin ISH
50%-epiboly margin ISH
30%-epiboly margin ISH
75%-epiboly margin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
lft1a145/a145 standard conditions Sphere blastodisc anatomical margin lft1 expression increased amount, abnormal
30%-epiboly margin lft1 expression increased amount, abnormal
Shield margin lft1 expression increased amount, abnormal
50%-epiboly margin lft1 expression increased amount, abnormal
Shield margin lft1 expression increased distribution, abnormal
50%-epiboly margin lft1 expression increased distribution, abnormal
30%-epiboly margin lft1 expression increased distribution, abnormal
Shield margin ndr1 expression increased amount, abnormal
50%-epiboly margin ndr1 expression increased amount, abnormal
30%-epiboly margin ndr1 expression increased amount, abnormal
Shield margin ndr1 expression increased distribution, abnormal
50%-epiboly margin ndr1 expression increased distribution, abnormal
30%-epiboly margin ndr1 expression increased distribution, abnormal
75%-epiboly axis lft1 expression increased amount, abnormal
75%-epiboly axis lft1 expression increased distribution, abnormal
75%-epiboly axis lft1 expression spatial pattern, abnormal
75%-epiboly axis ndr2 expression increased amount, abnormal
75%-epiboly axis ndr2 expression increased distribution, abnormal
75%-epiboly margin lft1 expression increased amount, abnormal
75%-epiboly margin lft1 expression increased distribution, abnormal
75%-epiboly margin lft1 expression spatial pattern, abnormal
lft1a145/a145 ; lft2a146/a146 standard conditions Sphere blastodisc anatomical margin lft1 expression increased amount, abnormal
50%-epiboly margin lft1 expression increased amount, abnormal
30%-epiboly margin lft1 expression increased amount, abnormal
30%-epiboly margin lft1 expression increased distribution, abnormal
30%-epiboly margin lft2 expression increased amount, abnormal
30%-epiboly margin lft2 expression increased distribution, abnormal
30%-epiboly margin lft2 expression spatial pattern, abnormal
30%-epiboly margin ndr1 expression increased amount, abnormal
30%-epiboly margin ndr1 expression increased distribution, abnormal
Shield margin lft1 expression increased amount, abnormal
50%-epiboly margin lft1 expression increased distribution, abnormal
Shield margin lft1 expression increased distribution, abnormal
50%-epiboly margin lft2 expression increased amount, abnormal
Shield margin lft2 expression increased amount, abnormal
50%-epiboly margin lft2 expression increased distribution, abnormal
Shield margin lft2 expression increased distribution, abnormal
50%-epiboly margin lft2 expression spatial pattern, abnormal
Shield margin lft2 expression spatial pattern, abnormal
50%-epiboly margin ndr1 expression increased amount, abnormal
Shield margin ndr1 expression increased amount, abnormal
50%-epiboly margin ndr1 expression increased distribution, abnormal
Shield margin ndr1 expression increased distribution, abnormal
75%-epiboly axis lft1 expression increased amount, abnormal
75%-epiboly axis lft1 expression increased distribution, abnormal
75%-epiboly axis lft1 expression spatial pattern, abnormal
75%-epiboly axis lft2 expression increased amount, abnormal
75%-epiboly axis lft2 expression increased distribution, abnormal
75%-epiboly axis lft2 expression spatial pattern, abnormal
75%-epiboly axis ndr2 expression increased amount, abnormal
75%-epiboly axis ndr2 expression increased distribution, abnormal
75%-epiboly endoderm sox17 expression increased amount, abnormal
75%-epiboly endoderm sox17 expression increased distribution, abnormal
75%-epiboly endoderm sox17 expression spatial pattern, abnormal
75%-epiboly margin lft1 expression increased amount, abnormal
75%-epiboly margin lft1 expression increased distribution, abnormal
75%-epiboly margin lft1 expression spatial pattern, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife